Methylated ea Motho NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene
Lebitso la sehlahisoa
HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Ho batho ba baholo, ho feta 10 8 intestinal epithelial lisele tse oela theoha lerako la mala letsatsi le letsatsi, 'me ba excreted le mantle a ka e khōlō mala peristalsis.Ka lebaka la lisele tsa hlahala li ka 'na tsa oela ka har'a mala a ho ata ho sa tloaelehang, setulo sa bakuli ba mala se na le lisele tse ngata tse kulang le likarolo tse sa tloaelehang tsa lisele, e leng motheo oa thepa bakeng sa ho lemoha mantle a tsitsitseng.Liphuputso li fumane hore phetoho ea methylation ea bahlahisi ba liphatsa tsa lefutso ke ketsahalo ea pele ea tumorigenesis, 'me lisebelisoa tsa liphatsa tsa lefutso tse fumanoang ho tloha lisampole tsa mantle tsa bakuli ba kankere ea colorectal li ka bonahatsa boteng ba kankere ka maleng pejana.
NDRG4, e tsejoang hape e le SMAP-8 le BDM1, ke e mong oa litho tse 'nè tsa NDRG gene family (NDRG1-4), e bontšitsoeng hore e amahanngoa le ho ata ha lisele, ho arohana, tsoelo-pele le khatello ea kelello.Ho netefalitsoe hore NDRG4 methylation ke biomarker e ka bang teng bakeng sa tlhahlobo e sa hlaseheng ea mofetše oa colorectal ka lisampole tsa mantle.
SEPT9 ke setho sa lelapa la lefutso la Septin, le nang le bonyane liphatsa tsa lefutso tse 13 tse kentseng sebaka se bolokiloeng sa GTPase se ka kopanyang liprotheine tse amanang le cytoskeleton, 'me e amahanngoa le karohano ea lisele le tumorigenesis.Boithuto bo fumane hore litaba tsa methylated Septin9 tsa liphatsa tsa lefutso li eketsoa lisampole tsa mantle ho tsoa ho bakuli ba nang le mofetše oa colorectal.
Liprotheine tse amanang le frizzled-related frizzled (sFRPs) ke liprotheine tse qhibilihang tseo e leng sehlopha sa bahanyetsi ba Wnt pathway ka lebaka la sebopeho sa bona se phahameng sa homology ho frizzled (Fz) receptor bakeng sa pontšo ea Wnt.Ho se sebetse ha mofuta oa SFRP ho fella ka ts'ebetso e sa laoleheng ea pontšo ea Wnt e amanang le mofetše oa colorectal.Hajoale, SFRP2 methylation ka setulong e ka sebelisoa e le biomarker e sa hlaseleng bakeng sa tlhahlobo ea mofets'e oa colorectal.
BMP3 ke setho sa TGF-B superfamily mme ka hona e bapala karolo ea bohlokoa ho nts'etsopele ea embryonic ka ho susumetsa le ho bopa popeho ea pele ea masapo.BMP3 ke hypermethylated ka mofetše oa mala 'me e ka sebelisoa e le letšoao la bohlokoa la hlahala.
SDC2 ke cell surface heparan sulfate proteoglycan e amehang taolong ea mekhoa e mengata ea fisioloji le ea mafu.Ho sebetsa 'meleng ho kenyelletsa ho ata ha lisele, ho arohana, ho khomarela, mokhatlo oa cytoskeletal, ho falla, pholiso ea maqeba, puisano ea lisele-matrix, angiogenesis;Mekhoa ea pathological e kenyelletsa ho ruruha le kankere.Boemo ba methylation ba lefutso la SDC2 liseleng tsa mofetše oa colorectal bo ne bo phahame haholo ho feta ba lisele tse tloaelehileng.
Channel
| Tšireletso ea karabelo A | VIC/HEX | mofuta oa methylated NDRG4 |
| ROX | mofuta oa methylated SEPT9 | |
| CY5 | taolo ya ka hare | |
| Tšireletso ea karabelo B | VIC/HEX | mofuta oa methylated SFRP2 |
| ROX | mofuta oa methylated BMP3 | |
| FAM | mofuta oa methylated SDC2 | |
| CY5 | taolo ya ka hare |
Tlhaloso
| Gene | Letshwao la Letshwao | Ct Boleng | Tlhaloso |
| NDRG4 | VIC (HEX) | Ct boleng≤38 | NDRG4 e ntle |
| Ct value>38 kapa unde | NDRG4 e mpe | ||
| SEPT9 | ROX | Ct boleng≤38 | SEPT9 e ntle |
| Ct value>38 kapa unde | SEPT9 e mpe | ||
| SFRP2 | VIC (HEX) | Ct boleng≤38 | SFRP2 e ntle |
| Ct value>38 kapa unde | SFRP2 e mpe |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Mohlala oa setulo |
| CV | ≤5.0% |
| Tse khethehileng | Ha ho na cross-reactivity le mofets'e oa sebete, mofets'e oa bile, mofets'e oa qoqotho le mofets'e oa matšoafo |
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Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).
