Kokoana ea Hepatitis A
Lebitso la sehlahisoa
HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kokoana-hloko ea Hepatitis A (HAV) ke sesosa se ka sehloohong sa lefu la sebete le matla la kokoana-hloko.Kokoana-hloko ena ke kokoana-hloko ea RNA e nang le maikutlo a nepahetseng 'me ke ea mofuta oa Hepadnavirus oa lelapa la Picornaviridae.Kokoana-hloko ea Hepatitis A, haholo-holo e fetisoang ke tsela ea mantle le molomo, e hanyetsanang le mocheso, li-acids, le metsoako e mengata ea tlhaho, e ka phela nako e telele likhetleng, metsing, mobung kapa mobung oa leoatle [1-3].E fetisoa ka ho ba le lijo kapa metsi a silafetseng, kapa a fetisoa ka ho toba ho tloha ho motho ho ea ho motho.Lijo tse amanang le HAV li kenyelletsa oyster le clams, fragole, raspberries, blueberries, dates, meroho e makhasi a matala le tamati e omisitsoeng ka halofo[4-6].
Channel
| FAM | HAV nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Mokelikeli: likhoeli tse 9, Lyophilized: likhoeli tse 12 |
| Mofuta oa Mohlala | Serum/ setuloana |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 2/μL |
| Tse khethehileng | Sebelisa lisebelisoa ho hlahloba likokoana-hloko tse ling tsa lefu la sebete tse kang hepatitis B, C, D, E, enterovirus 71, kokoana-hloko ea coxsackie, kokoana-hloko ea Epstein-Barr, norovirus, HIV le genome ea motho.Ha ho na cross-reactivity. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Real-Nako PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Mehlala ea serum
Khetho ea 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E lokela ho ntšoa ho ea ka litaelo.Molumo o khothaletsoang oa elution ke 80µL.
Khetho ea 2.
TIANMP Virus DNA/RNA Kit (YDP315-R) e entsoeng ke Tiangen Biotech (Beijing) Co., Ltd. E lokela ho ntšoa ho latela litaelo.Bolumo ea sampole e ntšitsoeng ke 140μL.Molumo o khothaletsoang oa elution ke 60µL.
2.Mehlala ea mantle
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E lokela ho ntšoa ho ea ka litaelo.Molumo o khothaletsoang oa elution ke 80µL.
