Kokoana ea Hepatitis E
Lebitso la sehlahisoa
HWTS-HP006 Hepatitis E Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kokoana-hloko ea Hepatitis E (HEV) ke kokoana-hloko ea RNA e bakang mathata a bophelo lefatšeng ka bophara.E na le mefuta e mengata ea baeti 'me e na le thepa ea ho tšela litšitiso tsa mefuta-futa.Ke e 'ngoe ea likokoana-hloko tsa bohlokoa ka ho fetisisa tsa zoonotic mme e baka kotsi e kholo ho batho le liphoofolo.HEV e fetisoa haholo ka fecal-oral transmission, hape e ka fetisoa ho ea holimo ka mahe a emolisitsoeng kapa mali.Har'a tsona, tseleng ea phetisetso ea mantle le molomo, metsi le lijo tse nang le tšoaetso ea HEV li hasane hohle, 'me kotsi ea tšoaetso ea HEV ho batho le liphoofolo e phahame[1-2].
Channel
| FAM | HEV nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Ho phunya 'metso |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Tse khethehileng | Kokoana-hloko ea Hepatitis E (HEV) ke kokoana-hloko ea RNA e bakang mathata a bophelo lefatšeng ka bophara.E na le mefuta e mengata ea baeti 'me e na le thepa ea ho tšela litšitiso tsa mefuta-futa.Ke e 'ngoe ea likokoana-hloko tsa bohlokoa ka ho fetisisa tsa zoonotic mme e baka kotsi e kholo ho batho le liphoofolo.HEV e fetisoa haholo ka fecal-oral transmission, hape e ka fetisoa ho ea holimo ka mahe a emolisitsoeng kapa mali.Har'a tsona, tseleng ea phetisetso ea mantle le molomo, metsi le lijo tse nang le tšoaetso ea HEV li hasane hohle, 'me kotsi ea tšoaetso ea HEV ho batho le liphoofolo e phahame[1-2]. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR System QuantStudio®5 Nako ea 'Nete ea PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E lokela ho ntšoa ho ea ka litaelo.Molumo o khothaletsoang oa elution ke 80µL.
Khetho ea 2
TIANMP Virus DNA/RNA Kit (YDP315-R) e entsoeng ke Tiangen Biotech (Beijing) Co., Ltd. E lokela ho ntšoa ka thata ho latela litaelo.Bolumo ea sampole e ntšitsoeng ke 140μL.Molumo o khothaletsoang oa elution ke 60µL.v
