Mefuta e 18 ea Vaerase ea Papilloma ea Motho e Kotsing e Phahameng (Typing ea 16/18) Kit ea ho Fumana Asiti ea Nucleic (Fluorescence PCR)
Lebitso la sehlahisoa
Mefuta e 18 ea Vaerase ea Papilloma ea Motho e Kotsing e Phahameng (Typing ea 16/18) Kit ea ho Fumana Asiti ea Nucleic (Fluorescence PCR)
Thuto ea mafu a seoa
Vaerase ea Papillomavirus ea Motho (HPV) ke ea lelapa la Papillomaviridae la kokoana-hloko ea DNA e nyenyane, e sa koaheloang, e chitja e nang le mela e 'meli, e bolelele ba genome ea lipara tse ka bang 8000 tsa motheo (bp). HPV e tšoaetsa batho ka ho kopana ka kotloloho kapa ka tsela e sa tobang le lintho tse silafetseng kapa phetiso ea thobalano. Vaerase ha e amane feela le moamoheli, empa hape e amana le lisele, 'me e ka tšoaetsa letlalo la motho le lisele tsa epithelial tsa mucosal, e leng se bakang mefuta e fapaneng ea li-papilloma kapa li-warts letlalong la motho le tšenyo e atileng ho epithelium ea tsela ea ho ikatisa.
Kankere ea molomo oa popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng haholo tsa tsela ea ho ikatisa ea basali. Lithuto li bontšitse hore tšoaetso e sa khaotseng ea HPV le tšoaetso e mengata ke e 'ngoe ea lisosa tse ka sehloohong tsa kankere ea molomo oa popelo. Hona joale, mekhoa ea phekolo e sebetsang e tsebahalang e ntse e haella bakeng sa kankere ea molomo oa popelo e bakoang ke HPV, kahoo ho sibolloa le ho thibeloa ha tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela kankere ea molomo oa popelo. Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea kliniki le kalafo ea kankere ea molomo oa popelo.
Mokero
| Sesebediswa sa ho Buffer sa Karabelo | Moqolotsi oa litaba | Mochini oa ho tima |
| 18HPV Reaction Buffer | FAM | Ha ho letho |
| VIC (HEX) | Ha ho letho | |
| ROX | Ha ho letho | |
| CY5 | Ha ho letho |
Li-parameter tsa Tekheniki
| Polokelo | ≤ -18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sampole ea moroto, Sampole ea swab ea molomo oa popelo ea basali, Sampole ea swab ea botšehali ea basali |
| CV | <5.0% |
| LoD | Moeli oa ho Lemoha (LoD): LoD ea kit ke Likopi tse 300/mL |
| Ho hlaka | Ha ho na karabelo e kopaneng le likokoana-hloko tse ling tse amanang le tsela ea ho ikatisa le DNA ea liphatsa tsa lefutso tsa motho. |
| Lisebelisoa tse Sebetsang | Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.), Mekhoa ea PCR ea Nako ea 'Nete ea Applied Biosystems 7500, Mekhoa ea PCR ea Nako ea 'Nete ea Applied Biosystems 7500 e Potlakileng, Mekhoa ea PCR ea Nako ea 'Nete ea QuantStudio®5, Mekhoa ea PCR ea Nako ea 'Nete ea LightCycler®480, Mekhoa ea ho Fumana PCR ea LineGene 9600 Plus (FQD-96A, theknoloji ea Hangzhou Bioer) le MA-6000 Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Tsamaiso ea PCR ea Nako ea 'Nete ea BioRad CFX96, Tsamaiso ea PCR ea Nako ea 'Nete ea BioRad CFX Opus 96. |
Phallo ea mosebetsi: Sheba Kit IFU bakeng sa lintlha tse qaqileng
Tharollo ea 1 (Ho ntšoa ha nucleic acid ka boiketsetso)
Tharollo ea 2: (Sesebelisoa sa ho Lokolla Sampole ea Macro le Micro-Test)
Li-reagent lia hlokahala empa ha lia fanoa
Sesebediswa sa ho Lokolla Sample Release Reagent sa Macro & Micro-Test (HWTS-3005-8), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Lintho tse sebelisoang lia hlokahala empa ha lia fanoa
1.5mL ea lipeipi tsa centrifuge tse se nang DNase/RNase, malebela a se nang DNase/RNase, li-strips tsa li-tube tse 8 bakeng sa PCR, centrifuge ea desktop, motsoako oa vortex ea desktop.
