Mefuta e 28 ea HPV Nucleic Acid Detection Kit (Fluorescence PCR)
Lebitso la sehlahisoa
Mefuta e 28 ea HPV Nucleic Acid Detection Kit (Fluorescence PCR)
Thuto ea mafu a seoa
Vaerase ea Papillomavirus ea Motho (HPV) ke ea lelapa la Papillomaviridae la kokoana-hloko ea DNA e nyenyane, e sa koaheloang, e chitja e nang le mela e 'meli, e bolelele ba genome ea lipara tse ka bang 8000 tsa motheo (bp). HPV e tšoaetsa batho ka ho kopana ka kotloloho kapa ka tsela e sa tobang le lintho tse silafetseng kapa phetiso ea thobalano. Vaerase ha e amane feela le moamoheli, empa hape e amana le lisele, 'me e ka tšoaetsa letlalo la motho le lisele tsa epithelial tsa mucosal, e leng se bakang mefuta e fapaneng ea li-papilloma kapa li-warts letlalong la motho le tšenyo e atileng ho epithelium ea tsela ea ho ikatisa.
Kankere ea molomo oa popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng haholo tsa tsela ea ho ikatisa ea basali. Lithuto li bontšitse hore tšoaetso e sa khaotseng ea HPV le tšoaetso e mengata ke e 'ngoe ea lisosa tse ka sehloohong tsa kankere ea molomo oa popelo. Hona joale, mekhoa ea phekolo e sebetsang e tsebahalang e ntse e haella bakeng sa kankere ea molomo oa popelo e bakoang ke HPV, kahoo ho sibolloa le ho thibeloa ha tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela kankere ea molomo oa popelo. Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea kliniki le kalafo ea kankere ea molomo oa popelo.
Mokero
| S/N | Mokero | Mofuta |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC(HEX) | Taolo ea Ka Hare | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
Li-parameter tsa Tekheniki
| Polokelo | ≤ -18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sampole ea moroto, Sampole ea swab ea molomo oa popelo ea basali, Sampole ea swab ea botšehali ea basali |
| CV | <5.0% |
| LoD | Likopi tse 300/mL |
| Ho hlaka | Ha ho na karabelo e kopaneng le likokoana-hloko tse ling tse amanang le tsela ea ho ikatisa le DNA ea liphatsa tsa lefutso tsa motho. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Applied Biosystems 7500 ea Nako ea 'Nete, Sistimi ea PCR ea Applied Biosystems 7500 e Potlakileng ea Nako ea 'Nete, Sistimi ea PCR ea QuantStudio®5 ea Nako ea 'Nete, Sistimi ea PCR ea SLAN-96P ea Nako ea 'Nete (Hongshi Medical Technology Co., Ltd.), Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete, Sistimi ea ho Fumana PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer), Sepalami sa Thermal sa Maqhubu sa MA-6000 sa Nako ea 'Nete (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete, Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete. |
Phallo ea mosebetsi: Sheba Kit IFU bakeng sa lintlha tse qaqileng
Tharollo ea 1 (Ho ntšoa ha nucleic acid ka boiketsetso)
Tharollo ea 2: (Sesebelisoa sa ho Lokolla Sampole ea Macro le Micro-Test)
Li-reagent lia hlokahala empa ha lia fanoa
Sesebediswa sa ho Lokolla Sample Release Reagent sa Macro & Micro-Test (HWTS-3005-8), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Lintho tse sebelisoang lia hlokahala empa ha lia fanoa
1.5mL ea li-tubes tse se nang DNase/RNase, malebela a se nang DNase/RNase, li-strips tse 8 tsa PCR, centrifuge ea tabletop, motsoako oa li-tabletop shaker.
