Motho EGFR Gene 29 Mutations

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha ka mokhoa o nepahetseng liphetoho tse tloaelehileng ho li-exons 18-21 tsa lefutso la EGFR ka lisampole ho tsoa ho bakuli ba mofetše oa matšoafo oa lisele tse seng nyane.


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HWTS-TM001A-EGFR ea Motho Gene 29 Mutations Detection Kit (Fluorescence PCR)

Epidemiology

Kankere ea matšoafo e fetohile sesosa se ka sehloohong sa lefu la mofetše lefatšeng ka bophara, e sokelang bophelo bo botle ba batho haholo.Kankere e seng e nyane ea lisele ea matšoafo e etsa karolo ea 80 lekholong ea bakuli ba mofetše oa matšoafo.Hona joale EGFR ke sepheo sa bohlokoa ka ho fetisisa sa limolek'hule bakeng sa phekolo ea kankere e seng e nyenyane ea lisele tsa matšoafo.Phosphorylation ea EGFR e ka khothaletsa kholo ea lisele tsa tumor, phapang, tlhaselo, metastasis, anti-apoptosis, le ho khothaletsa angiogenesis ea tumor.EGFR tyrosine kinase inhibitors (TKI) e ka thibela tsela ea EGFR ea pontšo ka ho thibela EGFR autophosphorylation, kahoo e thibela ho ata le ho arohana ha lisele tsa tumor, ho khothalletsa tumor cell apoptosis, ho fokotsa tumor angiogenesis, joalo-joalo, e le ho finyella phekolo e lebisitsoeng ho hlahala.Liphuputso tse ngata li bontšitse hore katleho ea phekolo ea EGFR-TKI e amana haufi-ufi le boemo ba phetoho ea liphatsa tsa lefutso tsa EGFR, 'me e ka thibela ka ho khetheha ho hōla ha lisele tsa tumor ka phetoho ea liphatsa tsa lefutso tsa EGFR.Mofuta oa EGFR o letsohong le lekhutšoanyane la chromosome 7 (7p12), e bolelele bo felletseng ba 200Kb mme e na le li-exons tse 28.Sebaka se fetotsoeng se fumaneha haholo ho li-exons 18 ho isa ho 21, li-codons 746 ho isa ho tse 753 tse hlakotsoeng ho li-account tsa exon 19 tse ka bang 45% mme phetoho ea L858R ho exon 21 e nka 40% ho isa ho 45%.Litaelo tsa NCCN bakeng sa Tlhahlobo le Kalafo ea Kankere e Seng e Nyenyane ea Matšoafo ea Cell e bolela ka ho hlaka hore tlhahlobo ea phetoho ea liphatsa tsa lefutso ea EGFR e hlokahala pele ho tsamaiso ea EGFR-TKI.Setsi sena sa liteko se sebelisetsoa ho tataisa tsamaiso ea litlhare tsa epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), le ho fana ka motheo oa meriana e ikemetseng bakeng sa bakuli ba nang le mofetše oa matšoafo oa sele e seng nyane.Setsi sena se sebelisoa feela bakeng sa ho lemoha liphetoho tse tloaelehileng mofuteng oa EGFR ho bakuli ba nang le kankere e seng e nyenyane ea kankere ea matšoafo.Liphetho tsa liteko ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le eona feela motheo oa kalafo e ikhethileng ea bakuli.Baoki ba lokela ho nahana ka boemo ba mokuli, matšoao a lithethefatsi, le kalafo Karabelo le matšoao a mang a tlhahlobo ea laboratori le lintlha tse ling li sebelisoa ho ahlola liphetho tsa liteko ka botlalo.

Channel

FAM IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer

Tekheniki Parameters

Polokelo Mokelikeli: ≤-18℃ Lefifing;Lyophilized: ≤30℃ Lefifing
Shelf-bophelo Mokelikeli: likhoeli tse 9;Lyophilized: likhoeli tse 12
Mofuta oa Mohlala lisele tse ncha tsa hlahala, karolo e hoammeng ea mafu, lisele tse kentsoeng ke parafine kapa karolo, plasma kapa serum.
CV 5.0%
LoD ho fumana tharollo ea nucleic acid ka tlas'a semelo sa 3ng/μL ea mofuta o hlaha, e khona ho bona sekhahla sa phetoho ea 1%.
Tse khethehileng Ha ho na cross-reactivity le mofuta oa hlaha oa genomic DNA ea motho le mefuta e meng e fetotsoeng
Lisebelisoa tse sebetsang Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7300 Real-Time PCR Systems

QuantStudio® 5 ea Nako ea 'Nete ea PCR Systems

Sistimi ea PCR ea LightCycler® 480 ea Nako ea 'Nete

Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96

Phallo ea Mosebetsi

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