Enterococcus e hanyetsanang le Vancomycin le Gene e hanyetsanang le Lithethefatsi
Lebitso la sehlahisoa
HWTS-OT090-Vancomycin-resistant Enterococcus le Gene Restant Gene Detection Kit (Fluorescence PCR)
Epidemiology
Resistance to Drug resistance e boetse e tsejoa e le ho hanyetsa lithethefatsi, ho bolela ho hanyetsa ha libaktheria ketsong ea lithethefatsi tse thibelang likokoana-hloko.Hang ha ho hanyetsa lithethefatsi ho etsahala, phello ea chemotherapy ea lithethefatsi e tla fokotseha haholo.Resistance to Drug (Resistance to Drug) e arotsoe ka bohanyetsi bo ka hare le bo fumanoang.Ho hanyetsa ka hare ho naha ho khethoa ke liphatsa tsa lefutso tsa baktheria tsa chromosomal, tse fetisitsoeng ho tloha molokong ho isa molokong, 'me li ke ke tsa fetoha.Ho hanyetsa ho fumanoa ho bakoa ke hore ka mor'a ho kopana le lithibela-mafu, libaktheria li fetola mekhoa ea tsona ea metabolism e le hore li se ke tsa bolaoa ke lithibela-mafu.
Liphatsa tsa lefutso tsa vancomycin tse hanyetsanang le VanA le VanB li fumanoa ho hanyetsa lithethefatsi, tseo har'a tsona VanA e bontšang maemo a phahameng a ho hanyetsa vancomycin le teicoplanin, VanB e bontša maemo a fapaneng a ho hanyetsa vancomycin, 'me e na le kutloelo-bohloko ho teicoplanin.Vancomycin e atisa ho sebelisoa ho phekola mafu a baktheria ea Gram-positive, empa ka lebaka la ho hlaha ha vancomycin-resistant enterococci (VRE), haholo-holo enterococcus faecalis le enterococcus faecium, e etsang karolo e fetang 90%, e tlisitse mathata a macha kalafong ea bongaka. .Hona joale, ha ho na lithethefatsi tse khethehileng tsa antibacterial bakeng sa phekolo ea VRE.Ho feta moo, VRE e ka boela ea fetisetsa liphatsa tsa lefutso tsa ho hanyetsa lithethefatsi ho enterococci kapa libaktheria tse ling tsa Gram-positive.
Channel
| FAM | Enterococci e hanyetsanang le Vancomycin (VRE): Enterococcus faecalis le Enterococcus faecium |
| VIC/HEX | Taolo ea ka hare |
| CY5 | vancomycin resistance gene VanB |
| ROX | vancomycin resistance gene VanA |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | sekhohlela, mali, moroto kapa likolone tse hloekileng |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| Tse khethehileng | Ha ho na cross-reactivity le likokoana-hloko tse ling tsa phefumoloho tse kang klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus Aeruginosa, jumollanischemia, hemophilus influenza. richia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, adenovirus ea ho hema, kapa lisampole li na le liphatsa tsa lefutso tse hananang le lithethefatsi CTX, mecA, SME, Mehlala ea SHV le TEM. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time |
Phallo ea Mosebetsi
Li-reagents tse khothalelitsoeng: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWBTS-300) .
