Mefuta e 28 ea Kotsi e Phahameng ea Papilloma Virus ea Motho (Typing ea 16/18) Nucleic Acid
Lebitso la sehlahisoa
HWTS-CC006A-28 Mefuta ea Kotsi e Phahameng ea Papilloma Virus ea Motho (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kankere ea popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng ka ho fetesisa tsa pampitšana ea pelehi ea mosali.Liphuputso li bontšitse hore tšoaetso ea HPV e phehellang le mafu a mangata ke e 'ngoe ea lisosa tse ka sehloohong tsa mofetše oa molomo oa popelo.Hajoale, liphekolo tse lemohuoeng tse sebetsang hantle li ntse li haella bakeng sa mofetše oa popelo o bakoang ke HPV, kahoo ho sibolloa kapele le thibelo ea tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela mofetše oa popelo.Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea bongaka le kalafo ea mofetše oa popelo.
Channel
| Reaction Mix | Channel | Mofuta |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Taolo ea ka hare | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Motsoako oa Cervical Swab, Vaginal Swab, Moroto |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300Likopi / mL |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 200μL ea letsoai le tloaelehileng ho emisa pellet mohatong oa 2.1, ebe ho ntšoa ho lokela ho etsoa ho latela ho litaelo tsa tšebeliso ea reagent ena ea ho ntša.Molumo o khothaletsoang oa elution ke 80μL.
Sehlahisoa se khothalelitsoeng: QIAamp DNA Mini Kit (51304) kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Eketsa 200μL ea letsoai le tloaelehileng ho emisa pellet mohatong oa 2.1, 'me joale ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena.Bolumo ea sampole e nkiloeng ea lisampole kaofela ke 200μL, 'me bophahamo ba molumo o khothaletsoang ke 100μL.
