Influenza A Virus Universal/H1/H3
Lebitso la sehlahisoa
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) ke microorganism e nyane ka ho fetisisa ea prokaryotic e nang le sebopeho sa sele mme ha e na lerako la sele lipakeng tsa libaktheria le livaerase.MP haholo e baka mafu a phefumoloho ho batho, haholo-holo ho bana le bacha.MP e ka etsa hore Mycoplasma hominis pneumonia, tšoaetso ea matšoafo a bana le atypical pneumonia.Matšoao a kliniki a fapane, haholo-holo ho khohlela ho matla, feberu, mohatsela, hlooho e opang, 'metso o bohloko, tšoaetso ea matšoafo a holimo le bronchopneumonia ke tsona tse atileng haholo.Bakuli ba bang ba ka 'na ba tšoaroa ke pneumonia e matla ka lebaka la tšoaetso e ka holimo ea matšoafo,' me khatello e matla ea ho hema kapa esita le lefu le ka hlaha.MP ke e 'ngoe ea likokoana-hloko tse tloaelehileng le tsa bohlokoa ho pneumonia e fumanoang ke sechaba (CAP), e ikarabellang bakeng sa 10% -30% ea CAP,' me karolo e ka eketseha ka makhetlo a 3-5 ha MP e atile.Lilemong tsa morao tjena, karolo ea MP ho CAP pathogens e eketsehile butle-butle.Kotsi ea tšoaetso ea Mycoplasma pneumoniae e eketsehile, 'me ka lebaka la lipontšo tsa eona tse sa tobang, ho bonolo ho ferekanngoa le sefuba sa baktheria le kokoana-hloko.Ka hona, tlhahlobo ea pele ea laboratori ke ea bohlokoa haholo bakeng sa tlhahlobo ea bongaka le kalafo.
Channel
| FAM | influenza Mofuta wa universal virus nucleic acid |
| VIC/HEX | ntaramane A H1 kokoana-hloko ea nucleic acid |
| ROX | ntaramane A H3 kokoana-hloko ea nucleic acid |
| CY5 | taolo ya ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | lesela la nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Tse khethehileng | Ha ho cross-reactivity le lisampole tse ling tsa phefumoloho tse kang Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, kokoana-hloko ea Coxsackie, kokoana-hloko ea Echo, Metapneuvirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, joalo-joalo le DNA ea genomic ea motho. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Nucleic Acid Extraction or Purification Reagent (YDP315-R) ka Tiangen Biotech (Beijing) Co.,Ltd.Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo tsa tšebeliso.Bolumo ea sampole e ntšitsoeng ke 140μL, 'me molumo o khothaletsoang oa elution ke 60μL.
Khetho ea 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo tsa tšebeliso.Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 80μL.
