Likokoana-hloko tse Phefumolohang li Kopanetsoe
Lebitso la sehlahisoa
HWTS-RT050-Mefuta e Tšeletseng ea Phefumoloho ea Pathogen Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Influenza, eo ka tlwaelo e tsejoang e le 'ntaramane', ke lefu le tšoaetsanoang le matla la ho hema le bakoang ke kokoana-hloko ea ntaramane, e tšoaetsanoang haholo 'me e fetisoa haholo ke ho khohlela le ho thimola.
Respiratory syncytial virus (RSV) ke kokoana-hloko ea RNA, ea lelapa la paramyxoviridae.
Human adenovirus (HAdV) ke kokoana-hloko ea DNA e likhoele habeli ntle le enfelopo.Bonyane 90 genotypes e fumanoe, e ka aroloa ka 7 subgenera AG.
Human rhinovirus (HRV) ke setho sa lelapa la Picornaviridae le mofuta oa Enterovirus.
Mycoplasma pneumoniae (MP) ke kokoana-hloko ea pathogenic e pakeng tsa baktheria le likokoana-hloko ka boholo.
Channel
| Channel | PCR-Mix A | PCR-Mix B |
| Mokhatlo oa FAM | IFV A | HADV |
| VIC/HEX Channel | HRV | IFV B |
| CY5 Channel | RSV | MP |
| ROX Channel | Taolo ea ka hare | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Oropharyngeal swab |
| Ct | ≤35 |
| LoD | 500Likopi / mL |
| Tse khethehileng | 1.Liphetho tsa liteko tsa cross-reactivity li bonts'itse hore ha ho na karabelo e fapaneng lipakeng tsa kit le coronavirus ea motho SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, mefuta ea vaerase ea Parainfluenza 1, 2, le 3, Chlamydia pneumoniae, human metapneumovirus, Enterovirus A, B, C, D, Epstein-Barr kokoana-hloko, kokoana-hloko ea Maselese, cytomegalovirus ea batho, Rotavirus, Norovirus, kokoana-hloko ea Mumps, Varicella-zoster virus, Legionella, Bordetella pertussis, Haemophilus influlococcus, aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans le human genomic nucleic acids. 2.Bokhoni ba ho thibela tšitiso: Mucin (60mg/mL), 10% (v/v) mali a motho, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (e nang le li-preservatives) (20mg/mL), beclomethasone ( 20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), le tobramycin (0.6mg/mL) li ile tsa khethoa bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bontšitse hore lintho tse sitisang ho tse ka holimo li ne li se na karabelo ea tšitiso ho liphello tsa liteko tsa likokoana-hloko. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler BioRad CFX96 Real-Nako PCR System, BioRad CFX Opus 96 Real-Nako PCR System |
Kakaretso ea Tharollo ea PCR
