SARS-CoV-2/influenza A /influenza B

Tlhaloso e Khutšoanyane:

Setsi sena se loketse tlhahlobo ea in vitro ea boleng ba SARS-CoV-2, ntaramane A le ntaramane B nucleic acid ea nasopharyngeal swab le oropharyngeal swab disampole tseo ho neng ho belaelloa hore ke ts'oaetso ea SARS-CoV-2, ntaramane A le ntaramane. B. E ka boela ea sebelisoa ho belaelloang pneumonia le linyeoeng tse belaelloang tsa cluster le bakeng sa ho lemoha le ho tsebahatsa SARS-CoV-2, influenza A le influenza B nucleic acid ka nasopharyngeal swab le oropharyngeal swab disampole tsa novel Coronavirus infection maemong a mang.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-RT148-SARS-CoV-2/ntaramane A /ntaramane B Nucleic Acid Combined Detection Kit (Fluorescence PCR)

Channel

Lebitso la Channel	PCR-Mix 1	PCR-Mix 2
Mokhatlo oa FAM	Mofuta oa ORF1ab	IVA
VIC/HEX Channel	Taolo ya ka hare	Taolo ya ka hare
CY5 Channel	N gene	/
ROX Channel	E gene	IVB

Tekheniki Parameters

Polokelo	-18℃
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	li-swabs tsa nasopharyngeal le li-oropharyngeal swabs
Sepheo	SARS-CoV-2 lipheo tse tharo (liphatsa tsa lefutso tsa Orf1ab, N le E)/ntaramane A /ntaramane B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2:300 Likopi/mL kokoana-hloko ea ntaramane A: 500 Copies/mL kokoana-hloko ea ntaramane ea B: 500 Likopi / mL
Tse khethehileng	a) Liphetho tsa liteko tsa sefapano li bonts'itse hore khiti e lumellana le coronavirus ea motho SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, kokoana-hloko ea ho hema ea syncytial A le B, parainfluenza virus 1, 2 le 3, rhinovirusA, B le C, adenovirus 1, 2, 3, 4, 5, 7 le 55, metapneumovirus ea motho, enterovirus A, B, C le D, kokoana-hloko ea motho ea cytoplasmic pulmonary, kokoana-hloko ea EB, kokoana-hloko ea maselese Human cytomegalovirus, rotavirus, norovirus, kokoana-hloko ea mumps, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniaeberculos, Casis bacterium, Casis, Casis, Mycoccus pneumoniae ta Ho ne ho se na karabelo e fapaneng lipakeng tsa Pneumocystis yersini le Cryptococcus neoformans. b) Bokhoni ba ho thibela tšitiso: khetha mucin (60mg/mL), 10% (V/V) mali a motho, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (e nang le preservative) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL) ), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) le tobramycin (0.6mg/mL) .Liphello li bontšitse hore lintho tse sitisang ho tse ka holimo li ne li se na karabelo ea tšitiso ho liphello tsa ho lemoha ha likokoana-hloko.
Lisebelisoa tse sebetsang	Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems SLAN^®-96P Litsamaiso tsa PCR tsa Nako ea 'Nete QuantStudio™ 5 ea Nako ea 'Nete ea PCR Systems LightCycler^®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System