Kokoana ea Ebola ea Zaire
Lebitso la sehlahisoa
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kokoana-hloko ea Ebola ke ea Filoviridae, e leng kokoana-hloko ea RNA ea khoele e le 'ngoe e sa arotsoeng.Livaerase ke likhoele tse telele tse bolelele ba virion bo bolelele ba 1000nm le bophara ba 100nm.Genome ea kokoana-hloko ea Ebola ke RNA e sa arotsoeng e nang le mefuta e sa tšoaneng e nang le boholo ba 18.9kb, e kenyelletsang liprotheine tse 7 tsa sebopeho le protheine e le 'ngoe e se nang sebopeho.Kokoana ea Ebola e ka aroloa ka mefuta e kang Zaire, Sudan, Bundibugyo, Tai Forest le Reston.Har'a bona, mofuta oa Zaire le oa Sudan ho tlalehiloe hore li bolaile batho ba bangata ka lebaka la tšoaetso.EHF (Ebola Hemorrhagic Fever) ke lefu le tšoaetsanoang le bakoang ke kokoana-hloko ea Ebola.Batho ba tšoaelitsoe haholo-holo ka ho kopana le maro a 'mele, ho tsoa le mantle a bakuli kapa liphoofolo tse nang le tšoaetso,' me lipontšo tsa kliniki ke haholo-holo feberu e hlahelang, ho tsoa mali le tšenyo ea litho tse ngata.EHF e na le sekhahla se phahameng sa lefu la 50% -90%.
Channel
| FAM | MP nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Serum e ncha, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Tse khethehileng | Sebelisa lisebelisoa ho leka litšupiso tse mpe tsa k'hamphani, liphetho li kopana le litlhoko. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System(FQD-96A, Hangzhou Bioer technology) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, le BioRad CFX Opus 96 Real-Time PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa se khothalelitsoeng: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) by Tiangen Biotech (Beijing) Co.,Ltd.E lokela ho ntšoa ho latela litaelo, 'me molumo o khothaletsoang oa sampole ke 140μL mme bophahamo ba molumo o khothaletsoang ke 60μL.
Khetho ea 2.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).It e lokela ho ntšoa ho latela litaelo.Bolumo ea sampole ea ho ntšoa ke 200μL, 'me bophahamo ba molumo o khothaletsoang ke 80μL.
Khetho ea 3.
Li-reagents tse khothalelitsoeng: Nucleic Acid Extraction Reagent (1000020261) le High Throughput Automated Sample Preparation System(MGISP-960) ke BGI li lokela ho ntšoa ho latela litaelo.Bophahamo ba ho ntšoa ke 160μL, 'me bophahamo ba molumo o khothaletsoang ke 60μL.
