Enterovirus Universal
Lebitso la sehlahisoa
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Lefu la leoto-molomo ke lefu le tšoaetsanoang le bakoang ke enteroviruses (EV).Hona joale, mefuta e 108 ea serotypes ea enteroviruses e fumanoe, e arotsoe ka lihlopha tse 'nè: A, B, C le D. Har'a tsona, enterovirus EV71 le CoxA16 ke likokoana-hloko tse ka sehloohong.Lefu lena hangata le hlaha ho bana ba ka tlase ho lilemo tse 5, 'me le ka baka herpes matsohong, maotong, molomong le likarolong tse ling.Palo e nyenyane ea bana e tla ba le mathata a kang myocarditis, pulmonary edema, le aseptic meningoencephalitis.
Channel
| FAM | EV RNA |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Oropharyngeal swab,Mokelikeli oa Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Likopi / mL |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500/7500 Fast Real-Time PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Khothaletsa Kit ea ho Ntša: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), e lokela ho ntšoa ka thata ho latela litaelo.Bolumo ea sampole ke 200 μL, bophahamo ba molumo o khothaletsoang ke 80µL.
Khetho ea 2.
Setsi sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), se lokela ho ntšoa ka thata ho latela litaelo.
Khetho3.
Setsi sa ho ntša se khothalelitsoeng: QIAamp Viral RNA Mini Kit (52904) kapa Nucleic Acid Extraction or Purification Kit (YDP315-R), se lokela ho ntšoa ho latela litaelo.Bolumo ea sampole ke 140 μL, bophahamo ba molumo o khothaletsoang ke 60µL.
