Clostridium difficile toxin A/B gene (C.diff)
Lebitso la sehlahisoa
HWTS-OT031A Nucleic Acid Detection Kit for Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Clostridium difficile (CD), gram-positive anaerobic sporogenic Clostridium difficile, ke e 'ngoe ea likokoana-hloko tse ka sehloohong tse bakang mafu a nosocomial mala.Ho ea ka meriana, hoo e ka bang 15% ~ 25% ea letšollo le amanang le likokoana-hloko, 50% ~ 75% ea lefu la ho ruruha le amanang le likokoana-hloko le 95% ~ 100% ea pseudomembranous enteritis e bakoa ke tšoaetso ea Clostridium difficile (CDI).Clostridium difficile ke pathogen e nang le maemo, ho kenyelletsa le mefuta e nang le chefo le mefuta e seng ea toxigenic.
Channel
| FAM | tcdAlefutso |
| ROX | tcdBlefutso |
| VIC/HEX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | setuloana |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Tse khethehileng | sebelisa setlolo sena ho lemoha likokoana-hloko tse ling tsa mala tse kang Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Sehlopha sa B Streptococcus, Clostridium difficile mefuta e seng ea pathogenic, Adenovirus, rotavirus, norovirus, kokoana-hloko ea ntaramane, le ntaramane. DNA, liphetho kaofela li mpe. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System(FQD-96A,HangzhouTheknoloji ea bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Kenya 180μL ea lysozyme buffer ho precipitate (hlapolla lysozyme ho 20mg/mL ka lysozyme diluent), pipette ho kopanya hantle, le ho sebetsa ka 37 ° C ka metsotso e fetang 30. Nka 1.5mL ea RNase / DNase-free centrifuge tube, le ho eketsa180μL ea taolo e ntle le taolo e mpe ka tatellano.Eketsa10μL ea taolo ea ka hare ho sampole e tla lekoa, taolo e nepahetseng, le taolo e mpe ka tatellano, le ho sebelisa Nucleic Acid Extraction kapa Purification Reagent (YDP302) ke Tiangen Biotech (Beijing) Co., Ltd. ka kopo latela ka tieo litaelo tsa tšebeliso bakeng sa mehato e itseng.Sebelisa DNase/RNase mahala H2O bakeng sa elution, 'me bophahamo ba modumo o khothaletsoang ke 100μL.
Khetho ea 2.
Nka 1.5mL ea RNase/DNase-free centrifuge tube, 'me u kenye 200μL ea taolo e ntle le taolo e mpe ka tatellano.Eketsa10μL ea taolo ea kahare ho sampole e tla lekoa, taolo e nepahetseng, le taolo e mpe ka tatellano, le ho sebelisa Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso, 'me bophahamo ba molumo o khothaletsoang ke 80μL.
