Enterovirus 71 (EV71)
Lebitso la sehlahisoa
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Lefu la leoto-molomo ke lefu le tšoaetsanoang le bakoang ke enteroviruses (EV).Hona joale, mefuta e 108 ea serotypes ea enteroviruses e fumanoe, e arotsoe ka lihlopha tse 'nè: A, B, C le D. Har'a tsona, enterovirus EV71 le CoxA16 ke likokoana-hloko tse ka sehloohong.Lefu lena hangata le hlaha ho bana ba ka tlase ho lilemo tse 5, 'me le ka baka herpes matsohong, maotong, molomong le likarolong tse ling.Palo e nyenyane ea bana e tla ba le mathata a kang myocarditis, pulmonary edema, le aseptic meningoencephalitis.
Channel
| FAM | EV71 |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Oropharyngeal swab,Mokelikeli oa Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Likopi / mL |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Litsamaiso tsa PCR tsa Nako ea 480 LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Recommended reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), 'me ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso.Mehlala e ntšitsoeng ke li-oropharyngeal swabs kapa lisampole tsa mokelikeli oa herpes ho tsoa ho bakuli ba bokelletsoeng setšeng.Eketsa li-swabs tse bokelletsoeng ho Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ka kotloloho, vortex 'me u kopanye hantle, beha mocheso oa kamore metsotso e 5, u li ntše ebe u li khelosa ho kopanya hantle, ho fumana RNA. ea mohlala ka mong.
Khetho ea 2.
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., 'me ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso.Bolumo ea sampole e ntšitsoeng ke 200µL, 'me bophahamo ba modumo e khothaletsoang ke 80µL.
Khetho3.
Sehlahisoa sa ho ntša se khothalelitsoeng: QIAamp Viral RNA Mini Kit (52904) ka QIAGEN kapa TIANamp Virus DNA/RNA Kit (YDP315-R), 'me mokoloto o lokela ho etsoa ho latela litaelo tse sebelisoang.Bolumo ea sampole e ntšitsoeng ke 140μL, 'me bophahamo ba modumo e khothaletsoang ke 60µL.
