Adenovirus Universal
Lebitso la sehlahisoa
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Human adenovirus (HAdV) ke ea mofuta oa Mammalian adenovirus, e leng kokoana-hloko ea DNA e nang le likarolo tse peli ntle le enfelopo.Li-Adenoviruses tse fumanoeng ho fihlela joale li kenyelletsa lihlopha tse 7 (AG) le mefuta ea 67, eo 55 serotypes e leng pathogenic ho batho.Har'a tsona, tse ka lebisang ho mafu a phefumoloho ke sehlopha sa B (Mofuta oa 3, 7, 11, 14, 16, 21, 50, 55), Sehlopha sa C (Mofuta oa 1, 2, 5, 6, 57) le Sehlopha sa E. (Mofuta oa 4), 'me o ka lebisa ho tšoaetso ea letšollo la mala ke Sehlopha sa F (Mofuta oa 40 le oa 41)[1-8].Mefuta e fapaneng e na le matšoao a fapaneng a kliniki, empa haholo-holo mafu a matšoafo a matšoafo.Mafu a phefumolohang a bakoang ke mafu a matšoafo a 'mele oa motho a etsa karolo ea 5% ~ 15% ea maloetse a phefumolohang lefatšeng ka bophara, le 5% -7% ea mafu a lefats'e a ho hema bongoaneng[9].Adenovirus e jele setsi libakeng tse ngata tse fapaneng 'me e ka tšoaetsoa selemo ho pota, haholo-holo libakeng tse nang le batho ba bangata, tse atisang ho hlaseloa ke libaka, haholo-holo likolong le likampong tsa sesole.
Channel
| FAM | adenovirus bokahohlengasiti ea nucleic |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Nasopharyngeal swab,Ho phunya 'metso |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Likopi / mL |
| Tse khethehileng | a) Lekola litšupiso tse mpe tsa k'hamphani e hlophisitsoeng ke kit, 'me sephetho sa liteko se kopana le litlhoko. b) Sebelisa sephutheloana sena ho lemoha 'me ha ho na cross-reactivity le likokoana-hloko tse ling tsa phefumoloho (joalo ka kokoana-hloko ea Influenza A, Influenza B, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, joalo-joalo) kapa baktheria (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, joalo-joalo). |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana SistimisFQD-96A, HangzhouTheknoloji ea bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Nako PCR Systems, BioRad CFX Opus 96 Real-Nako PCR Systems |
Phallo ea Mosebetsi
(1) Sesebelisoa sa ho ntša se khothalelitsoeng:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ho ntšoa ho lokela ho etsoa ho latela litaelo.Mohlala o ntšitsoeng ke bakuli'nasopharyngeal swab kapa disampole tsa 'metso tse bokeletsoeng setšeng.Eketsa lisampole ka har'a sampole ea tokollo e entsoeng ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex ho kopanya hantle, beha mocheso oa kamore ka metsotso e 5, e ntšoe ebe o khelosa le ho kopanya hantle ho fumana DNA ea mohlala ka mong.
(2) Regent e khothalletsoang ea ho ntša:Macro & Micro-Test Kokoana-hloko DNA/RNA Kit(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).opereishene e lokela ho etsoa ka thata ho latela litaelo.Bolumo ea sampole e ntšitsoeng ke 200μL, le hokgothaletswa elution bophahamo ba modumois80μL.
(3) Sehlahisoa sa ho ntša se khothalelitsoeng: Nucleic Acid Extraction kapa Purification Reagent (YDP315) ke Tiangen Biotech (Beijing) Co.,Ltd., tseopereishene e lokela ho etsoa ka tieo ho latela litaelo.Bolumo ea sampole e ntšitsoeng ke 200μL, le hokgothaletswa elution bophahamo ba modumois80μL.
