Kokoana ea Hepatitis B RNA
Lebitso la sehlahisoa
HWTS-HP007 Hepatitis B Virus RNA Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Qho lemoha ho sa bonahaleng ha maemo a HBV-RNA ho serum ho ka lekola boemo ba HBV cccDNA ho hepatocyte, 'me ka nako e ts'oanang ho qoba ho baka tšenyo e kholo ho bakuli.Ho bohlokoa haholo bakeng sa tlhahlobo e thusang ea ts'oaetso ea kokoana-hloko ea hepatitis B (HBV) le tlhahlobo ea katleho le polelo ea ho tlosoa ha lithethefatsi ea NAs antiviral therapy ho bakuli ba CHB.
Channel
| FAM | HBV-RNA |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | serum |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 100 likopi / mL |
| Tse khethehileng | Liphetho tsa liteko tsa tšitiso li bonts'a hore ha ho na litlamorao ho qeto ea HBV RNA ha bongata ba bilirubin ka serum bo sa fete 168.2μmol/mL, khatello ea hemoglobin e hlahisoang ke hemolysis ha e fete 130g/L, le boemo ba lipids maling ha bo fete 65mmol/mL;ha ho na liphello tse mabapi le boikemisetso ba HBV RNA ha kakaretso ea IgG ka serum e sa fete 5mg / mL.Liphetho tsa liteko tsa cross-reactivity li bonts'a hore ha ho na likarabelo lipakeng tsa kit ena le livaerase tse ling kapa libaktheria tse fumanoeng ke nucleic acid ka lisampole tsa mali (vaerase ea hepatitis C, cytomegalovirus ea motho, kokoana-hloko ea epstein-barr, kokoana-hloko ea immunodeficiency virus, kokoana-hloko ea hepatitis A, syphilis). , kokoana-hloko ea herpes ea mofuta oa 6, herpes simplex kokoana-hloko ea mofuta oa 1, kokoana-hloko ea herpes simplex mofuta oa 2, kokoana-hloko ea ntaramane A, propionibacterium acnes, staphylococcus aureus, candida albicans) le liphatsa tsa lefutso tsa batho;ka mor'a hore bakuli ba nke lamivudine, telbivudine, adefovir dipivoxil le entecavir, ha kokoana-hloko ea kokoana-hloko ea lefu la hepatitis B 'meleng e le ka holim'a bonyane ba LoD ea kit, kit e ntse e ka fumanoa ka katleho. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCRApplied Biosystems 7500 Fast Real-Time PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) |
Phallo ea Mosebetsi
Khetho ea 1.
Viral DNA/RNA Isolation Kit (NA007-2) by GenMag Biotechnology Co., Ltd. Mehato e khethehileng ea ho ntša ka kopo ka kopo latela ka tieo buka ea litaelo.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ho latela bukana ea litaelo, molumo oa sampole o ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 80μL.
