Human Cytomegalovirus (HCMV) Nucleic Acid

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho etsa qeto ea boleng ba li-nucleic acid ka lisampole ho kenyelletsa serum kapa plasma ho tsoa ho bakuli ba belaelloang hore ba na le tšoaetso ea HCMV, e le ho thusa ho fumana tšoaetso ea HCMV.


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-UR008A-Human cytomegalovirus (HCMV) nucleic acid kit (Fluorescence PCR)

Epidemiology

Human cytomegalovirus (HCMV) ke setho se nang le genome e kholo ka ho fetisisa lelapeng la herpes virus mme e ka kenyelletsa liprotheine tse fetang 200.HCMV e thibetsoe ka mokhoa o fokolang sebakeng sa eona se amohelang batho, 'me ho ntse ho se na mohlala oa liphoofolo oa tšoaetso ea eona.HCMV e na le potoloho e liehang le e telele ea ho ikatisa ho theha 'mele oa ho kenyelletsa intranuclear, le ho etsa hore ho be le 'mele oa ho kenyelletsa perinuclear le cytoplasmic le ho ruruha ha sele (lisele tse kholo), ka hona lebitso.Ho ea ka mefuta e fapaneng ea genome le phenotype ea eona, HCMV e ka aroloa ka mefuta e sa tšoaneng, eo har'a eona ho nang le mefuta e itseng ea li-antigenic, tseo, leha ho le joalo, li se nang bohlokoa ba kliniki.

Ts'oaetso ea HCMV ke ts'oaetso ea tsamaiso, eo ka mokhoa oa bongaka e amang litho tse ngata, e na le matšoao a rarahaneng le a sa tšoaneng, hangata e khutsitse, 'me e ka etsa hore bakuli ba' maloa ba be le liso tsa litho tse ngata tse akarelletsang retinitis, hepatitis, pneumonia, encephalitis, colitis, monocytosis le thrombocytopenic. purpura.Tšoaetso ea HCMV e tloaelehile haholo 'me e bonahala e ata lefatšeng ka bophara.E atile haholo ho baahi, ka sekhahla sa ts'oaetso ea 45-50% le ho feta 90% linaheng tse tsoetseng pele le tse tsoelang pele, ka ho latellana.HCMV e ka robala 'meleng nako e telele.Hang ha tšireletso ea 'mele e fokotsehile, kokoana-hloko e tla kenngoa ts'ebetsong ho baka mafu, haholo-holo ho tšoaetsoa hangata ho bakuli ba leukemia le bakuli ba transplant,' me e ka baka necrosis ea litho tsa 'mele le ho beha bophelo ba bakuli kotsing maemong a boima.Ntle le ho tsoala lesea le shoeleng, ho senyeheloa ke mpa le ho beleha pele ho nako ka tšoaetso ea intrauterine, cytomegalovirus e ka boela ea baka mathata a congenital, kahoo tšoaetso ea HCMV e khona ho ama tlhokomelo ea bakhachane le ba pelehi le boleng ba baahi.

Channel

FAM HCMV DNA
VIC(HEX) Taolo ea ka hare

Tekheniki Parameters

Polokelo

Mokelikeli: ≤-18℃ Lefifing

Shelf-bophelo

Likhoeli tse 12

Mofuta oa Mohlala

Mohlala oa Serum, Mohlala oa Plasma

Ct

≤38

CV

≤5.0%

LoD

50 Likopi/karabelo

Tse khethehileng

Ha ho na cross-reactivity le kokoana-hloko ea hepatitis B, kokoana-hloko ea hepatitis C, kokoana-hloko ea papilloma ea motho, kokoana-hloko ea herpes simplex mofuta oa 1, herpes simplex virus mofuta oa 2, lisampole tse tloaelehileng tsa serum ea motho, joalo-joalo.

Lisebelisoa tse Sebetsang:

E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Nako ea 'Nete ea PCR Systems

Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P

Sesebelisoa sa PCR sa LightCycler®480 Real-Time

LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi

MA-6000 Real-Nako Quantitative Thermal Cycler

Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96

BioRad CFX Opus 96 Real-Nako PCR System

Phallo ea Mosebetsi

HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ho ntšoa ho lokela ho ntšoa ho latela litaelo.Bolumo ea sampole ea ho ntšoa ke 200μL, 'me bophahamo ba molumo o khothaletsoang ke 80μL.

Sehlahisoa se khothalelitsoeng: QIAamp DNA Mini Kit (51304), Nucleic Acid Extraction or Purification Reagent(YDP315) by Tiangen Biotech(Beijing) Co.,Ltd.e lokela ho ntšoa ho ea ka litaelo tsa ho ntšoa, 'me bophahamo ba molumo o khothaletsoang ke 200 μL le bophahamo ba molumo o khothaletsoang ke 100 μL.


  • E fetileng:
  • E 'ngoe:

  • Ngola molaetsa wa hao mona mme o re romele wona