Sete ea ho lemoha nucleic acid ea mofuta oa 1/2 oa kokoana-hloko ea Herpes simplex (HSV1/2)

Sete ea ho lemoha nucleic acid ea mofuta oa Herpes simplex 1/2, (HSV1/2) Setšoantšo se Hlahisitsoeng

Tlhaloso e Khutšoanyane:

Sete ena e sebelisetsoa ho lemoha ka hare ho vitro mofuta oa 1 oa kokoana-hloko ea Herpes Simplex (HSV1) le mofuta oa 2 oa kokoana-hloko ea Herpes Simplex (HSV2) ho thusa ho hlahloba le ho phekola bakuli ba belaelloang hore ba na le tšoaetso ea HSV.

Romella imeile ho rona

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

Sete ea ho lemoha nucleic acid ea mofuta oa 1/2 oa kokoana-hloko ea Herpes simplex (HSV1/2)

Thuto ea mafu a seoa

Mafu a tšoaetsanoang ka thobalano (STD) e ntse e le e 'ngoe ea litšokelo tse kholo ts'ireletsong ea bophelo bo botle ba sechaba lefatšeng ka bophara. Mafu a joalo a ka lebisa ho hloka thari, ho beleha lesea pele ho nako, hlahala le mathata a fapaneng a tebileng. Ho na le mefuta e mengata ea likokoana-hloko tsa STD, ho kenyeletsoa libaktheria, livaerase, chlamydia, mycoplasma le spirochetes, tseo har'a tsona Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, le Ureaplasma urealyticum li atileng haholo.

Herpes ea botona kapa botšehali ke lefu le tloaelehileng le tšoaetsanoang ka thobalano le bakoang ke HSV2, e tšoaetsanoang haholo. Lilemong tsa morao tjena, ho ata ha herpes ea botona kapa botšehali ho eketsehile haholo, 'me ka lebaka la keketseho ea boitšoaro bo kotsi ba thobalano, sekhahla sa ho fumanoa ha HSV1 ho herpes ea botona kapa botšehali se eketsehile 'me ho tlalehiloe hore se holimo ho 20%-30%. Tšoaetso ea pele ea vaerase ea herpes ea botona kapa botšehali hangata e khutsitse ntle le matšoao a hlakileng a kliniki ntle le herpes ea lehae ka har'a mucosa kapa letlalo la bakuli ba 'maloa. Kaha herpes ea botona kapa botšehali e khetholloa ka ho tsoa ha vaerase bophelo bohle le ho ba le tšekamelo ea ho khutla hape, ho bohlokoa ho hlahloba likokoana-hloko kapele kamoo ho ka khonehang le ho thibela phetiso ea eona.

Mokero

FAM	HSV1
CY5	HSV2
VIC(HEX)	Taolo ea Ka Hare

Li-parameter tsa Tekheniki

Polokelo	Metsi: ≤-18℃ Lefifing
Nako ea ho phela	Likhoeli tse 12
Mofuta oa Sampole	maro a tsoang mothapong oa urethra, maro a tsoang molomong oa popelo
Ct	≤38
CV	≤5.0%
LoD	Likopi/karabelo tse 50
Ho hlaka	Ha ho na cross-reactivity le likokoana-hloko tse ling tsa STD tse kang Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, le Ureaplasma urealyticum.
Lisebelisoa tse Sebetsang	E ka bapisoa le lisebelisoa tse tloaelehileng tsa PCR tse khanyang tse rekisoang 'marakeng. Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio^®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler^®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete

Mokgwa wa mosebetsi

Ho hlokahala li-reagent empa ha lia fanoa

Sesebediswa sa ho Lokolla Sample Release Reagent (HWTS-3005-8), Kit ya Viral DNA/RNA ya Macro le Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro le Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kit ya ho Tlosa kapa ho Hloekisa Nucleic Acid (YDP315) ho tswa ho Tiang Biotech (Beijing) Co., Ltd.

Lisebelisoa tse hlokahalang empa ha lia fanoa

Li-tubes tsa centrifuge tse se nang DNase/RNase tsa 1.5 mL, li-tips tse se nang DNase/RNase, ethanol e feletseng, pipettor, benchtop centrifuge, benchtop vortex mixer.

E fetileng: Mefuta e 14 ea HPV Nucleic Acid Type

E 'ngoe: Sete ea ho Fumana Mycoplasma Hominis Nucleic Acid (Fluorescence PCR)

Ngola molaetsa oa hau mona 'me u o romelle ho rona