Herpes Simplex Virus Type 1/2，(HSV1/2) Nucleic Acid

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba Herpes Simplex Virus Type 1 (HSV1) le Herpes Simplex Virus Type 2 (HSV2) ho thusa ho hlahloba le ho phekola bakuli ba belaelloang hore ba na le tšoaetso ea HSV.

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Lintlha tsa Sehlahisoa

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Lebitso la sehlahisoa

HWTS-UR018A-Herpes simplex virus mofuta 1/2, (HSV1/2) nucleic acid e lemoha lisebelisoa (Fluorescence PCR)

Epidemiology

Mafu a tšoaetsanoang ka thobalano (STD) e ntse e le e 'ngoe ea litšokelo tse kholo ho ts'ireletseho ea bophelo bo botle ba sechaba lefatšeng ka bophara.Mafu a joalo a ka baka ho hloka thari, ho beleha lesea pele ho nako, hlahala le mathata a fapaneng a tebileng.Ho na le mefuta e mengata ea likokoana-hloko tsa STD, ho akarelletsa le libaktheria, likokoana-hloko, chlamydia, mycoplasma le spirochetes, tseo har'a tsona Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, le Ureaplasma urealyticum e tloaelehileng.

Genital herpes ke lefu le tloaelehileng la thobalano le bakoang ke HSV2, e tšoaetsanoang haholo.Lilemong tsa morao tjena, ts'oaetso ea herpes ea thobalano e eketsehile haholo, 'me ka lebaka la ho eketseha ha boitšoaro bo kotsi ba ho kopanela liphate, sekhahla sa ho lemoha HSV1 ho genital herpes se eketsehile' me ho tlalehoa hore se ka holimo ho 20% -30%.Tšoaetso ea pele e nang le kokoana-hloko ea genital herpes hangata e khutsitse ntle le matšoao a hlakileng a kliniki ntle le herpes ea sebakeng sa mucosa kapa letlalo la bakuli ba seng bakae.Kaha herpes ea genital e tšoauoa ka ho tšolloa ha kokoana-hloko ea bophelo bohle le ho ba le tšekamelo ea ho khutlela hape, ho bohlokoa ho hlahloba likokoana-hloko kapele kamoo ho ka khonehang le ho thibela phetiso ea eona.

Channel

FAM	HSV1
CY5	HSV2
VIC(HEX)	Taolo ea ka hare

Tekheniki Parameters

Polokelo	Mokelikeli: ≤-18℃ Lefifing
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	secretions urethral, secretions cervical
Ct	≤38
CV	≤5.0%
LoD	50 Likopi/karabelo
Tse khethehileng	Ha ho na cross-reactivity le likokoana-hloko tse ling tsa STD tse kang Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, le Ureaplasma urealyticum.
Lisebelisoa tse sebetsang	E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng. Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler^®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System

Phallo ea Mosebetsi

Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent(HWTS-3005-8).Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo.

Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo.Molumo o khothaletsoang oa elution e lokela ho ba 80 μL.

Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction kapa Purification Reagent(YDP315) ke Tiangen Biotech(Beijing) Co.,Ltd.Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo.Molumo o khothaletsoang oa elution e lokela ho ba 80 μL.

E fetileng: Likokoana-hloko tse Phefumolohang li Kopanetsoe

E 'ngoe: Mycoplasma Hominis Nucleic Acid

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