Adenovirus Universal
Lebitso la sehlahisoa
HWTS-RT017A Kit ea ho Fumana Adenovirus Universal Nucleic Acid (Fluorescence PCR)
Thuto ea mafu a seoa
Adenovirus ea motho (HAdV) ke ea mofuta oa adenovirus ea Mammalian, e leng vaerase ea DNA e nang le mela e 'meli e sa koaheloang. Li-adenovirus tse fumanoeng ho fihlela joale li kenyelletsa lihlopha tse 7 tse nyenyane (AG) le mefuta e 67, eo ho eona li-serotype tse 55 li bakang mafu ho batho. Har'a tsona, tse ka lebisang tšoaetsong ea phefumoloho ke sehlopha sa B (Mefuta ea 3, 7, 11, 14, 16, 21, 50, 55), Sehlopha sa C (Mefuta ea 1, 2, 5, 6, 57) le Sehlopha sa E (Mofuta oa 4), 'me tse ka lebisang tšoaetsong ea letšollo la mala ke Sehlopha sa F (Mefuta ea 40 le 41) [1-8]. Mefuta e fapaneng e na le matšoao a fapaneng a kliniki, empa haholo-holo tšoaetso ea phefumoloho. Mafu a phefumoloho a bakoang ke tšoaetso ea phefumoloho ea 'mele oa motho a etsa 5% ~ 15% ea mafu a phefumoloho lefatšeng ka bophara, le 5% - 7% ea mafu a phefumoloho a bana lefatšeng ka bophara [9]. Adenovirus e atile haholo libakeng tse fapaneng 'me e ka tšoaetsoa selemo ho pota, haholo-holo libakeng tse tletseng batho ba bangata, tse atisang ho ba le seoa sa lehae, haholo-holo likolong le likampong tsa sesole.
Mokero
| FAM | adenovirus ea bokahohleasiti ea nucleic |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Swab ea nasopharyngeal，Sesepa sa 'metso |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 300/mL |
| Ho hlaka | a) Leka litšupiso tse fosahetseng tsa k'hamphani tse tloaelehileng ka kit, 'me sephetho sa teko se fihlela litlhoko. b) Sebelisa kit ena ho lemoha 'me ha ho na mokhoa oa ho kopana le likokoana-hloko tse ling tsa ho hema (joalo ka kokoana-hloko ea Influenza A, kokoana-hloko ea Influenza B, kokoana-hloko ea Respiratory syncytial, kokoana-hloko ea Parainfluenza, Rhinovirus, Human metapneumovirus, jj.) kapa libaktheria (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, jj.). |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Netes (FQD-96AHangzhouTheknoloji ea Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Mekhoa ea PCR ea BioRad CFX96 ea Nako ea 'Nete, Mekhoa ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
(1) Reagent e kgothaletswang ya ho ntsha:Setlolo sa ho Lokolla Sample Release Reagent sa Macro & Micro-Test (HWTS-3005-8). Ho ntšoa ha sampole ho lokela ho etsoa ho latela litaelo. Sampole e ntšitsoeng ke bakuli.'disampole tsa swab ya nasopharyngeal kapa swab ya mmetso tse bokelletsweng sebakeng seo. Kenya disampole ka hara reagent ya ho lokolla sampole ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex ho di kopanya hantle, di behe mochesong wa kamore metsotso e 5, di ntshe ebe o di fetola le ho di kopanya hantle ho fumana DNA ya sampole ka nngwe.
(2) Reagent e kgothaletswang ya ho ntsha:Teko ea Macro le Micro E atileng haholo Kiti ea DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).ts'ebetso e lokela ho etsoa ho latela litaelo ka tieo. Bophahamo ba sampole e ntšitsoeng ke 200μL, lebophahamo ba tharollo bo khothaletsoangis80μL.
(3) Reagent e kgothalletswang ya ho ntsha: Nucleic Acid Extract kapa Reagent ya ho Hloekisa (YDP)315) ke Tiangen Biotech (Beijing) Co.,Ltd., eTs'ebetso e lokela ho etsoa ho latela litaelo ka tieo. Bophahamo ba sampole e ntšitsoeng ke 200μL, lebophahamo ba tharollo bo khothaletsoangis80μL.
