Mycobacterium Tuberculosis Nucleic Acid le Rifampicin(RIF)，Isoniazid Resistance(INH)
Lebitso la sehlahisoa
HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, haufinyane joalo ka Tubercle bacillus (TB), ke baktheria ea pathogenic e bakang lefuba.Hajoale, litlhare tsa moleng oa pele tse atisang ho sebelisoa tse thibelang lefuba li kenyelletsa isoniazid, rifampicin le ethambutol, joalo-joalo. Litlhare tse thibelang lefuba tsa moeli oa bobeli li kenyelletsa fluoroquinolones, amikacin le kanamycin, joalo-joalo. Litlhare tse ncha tse entsoeng ke linezolid, bedaquiline le delamani, joalo-joalo. Leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea lithethefatsi tse thibelang lefuba le litšobotsi tsa sebopeho sa lerako la sele ea lefuba la mycobacterium, lefuba la mycobacterium le hlahisa ho hanyetsa lithethefatsi khahlanong le lithethefatsi tse thibelang lefuba, tse tlisang mathata a tebileng ho thibela le ho phekola lefuba.
Channel
| Lebitso la sepheo | Moqolotsi oa litaba | Quencher | ||
| Reaction BufferA | Reaction BufferB | Reaction BufferC | ||
| rpoB 507-514 | rpoB 513-520 | IS6110 | FAM | Ha ho letho |
| rpoB 520-527 | rpoB 527-533 | / | CY5 | Ha ho letho |
| / | / | Taolo ya ka hare | HEX(VIC) | Ha ho letho |
| Reaction BufferD | Moqolotsi oa litaba | Quencher |
| Sebaka sa papatso sa InhA -15C>T, -8T>A, -8T>C | FAM | Ha ho letho |
| KatG 315 codon 315G>A,315G>C | CY5 | Ha ho letho |
| Sebaka sa papatso sa AhpC -12C>T, -6G>A | ROX | Ha ho letho |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | sekhohlela |
| CV | ≤5.0% |
| LoD | The LOD of mycobacterium tuberculosis national reference is 50 bacteria/mL.LOD ea mofuta oa naha o hananang le rifampicin ke 2×103baktheria/mL, le LoD ea mofuta o fetotsoeng ke 2×103libaktheria/mL.LOD ea mofuta o hlaha oa baktheria e hanyetsanang le isoniazid ke 2x103baktheria/mL, le The LoD ea libaktheria tse fetohileng ke 2x103libaktheria/mL. |
| Tse khethehileng | Liphetho tsa liteko tsa sefapano li bonts'itse hore ho ne ho se na karabelo ea sefapano ho sibolloeng ha lefutso la motho, likokoana-hloko tse ling tse se nang lefuba tsa mycobacteria le likokoana-hloko tsa nyumonia ka khithi ena;Ho ne ho se na karabelo e fapaneng e fumanoeng libakeng tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le lithethefatsi ho lefuba la Mycobacterium le hlaha. |
| Lisebelisoa tse sebetsang | SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.), Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96, Theknoloji ea Hangzhou Bioer QuantGene 9600 Real-Nako PCR System, QuantStudio®Sistimi ea 5 ea Nako ea 'Nete ea PCR.
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Kakaretso ea Tharollo ea PCR
