Mycobacterium Tuberculosis Rifampicin Resistance

Tlhaloso e Khutšoanyane:

Setsi sena se loketse ho lemoha boemo ba phetoho ea homozygous sebakeng sa 507-533 amino acid codon ea gene ea rpoB e bakang Mycobacterium tuberculosis rifampicin resistance.

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HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Resistance Detection Kit (Fluorescence PCR)

Epidemiology

Rifampicin esale e sebelisoa haholo kalafong ea bakuli ba lefuba la matšoafo ho tloha bofelong ba lilemo tsa bo-1970, 'me e na le phello e kholo.E bile khetho ea pele ea ho khutsufatsa chemotherapy ea bakuli ba lefuba la matšoafo.Ho hanyetsa ha Rifampicin ho bakoa haholo-holo ke phetoho ea mofuta oa rpoB.Le hoja litlhare tse ncha tse thibelang lefuba li lula li tsoa, 'me katleho ea bongaka ea bakuli ba lefuba la matšoafo le eona e tsoetse pele ho ntlafala, ho ntse ho e-na le khaello e itseng ea lithethefatsi tse thibelang lefuba,' me ketsahalo ea tšebeliso e sa utloahaleng ea tšebeliso ea lithethefatsi litleleniki e batla e phahame.Ho hlakile hore lefuba la Mycobacterium ho bakuli ba nang le lefuba la matšoafo le ke ke la bolaoa ka ho feletseng ka nako e loketseng, e leng se qetellang se lebisa likarolong tse fapaneng tsa ho hanyetsa lithethefatsi 'meleng oa mokuli, ho lelefatsa nako ea lefu lena, le ho eketsa kotsi ea lefu la mokuli.Setsi sena se loketse tlhahlobo ea tlatsetso ea tšoaetso ea lefuba la Mycobacterium le ho lemoha lefutso la rifampicin, e leng ho thusang ho utloisisa khanyetso ea meriana ea lefuba la mycobacterium le tšoaelitsoeng ke bakuli, le ho fana ka mekhoa e thusang bakeng sa tataiso ea meriana ea bongaka.

Epidemiology

Lebitso la sepheo			Moqolotsi oa litaba	Quencher
Reaction BufferA	Reaction BufferB	Reaction BufferC	Moqolotsi oa litaba	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	Ha ho letho
rpoB 520-527	rpoB 527-533	/	CY5	Ha ho letho
/	/	Taolo ya ka hare	HEX(VIC)	Ha ho letho

Tekheniki Parameters

Polokelo	≤-18℃ Lefifing
Shelf-bophelo	Likhoeli tse 9
Mofuta oa Mohlala	Sekhohlela
CV	＜5％
LoD	mofuta oa hlaha o hananang le rifampicin: 2x10³libaktheria/mL phetoho ea homozygous: 2x10³libaktheria/mL
Tse khethehileng	E lemoha lefuba la mycobacterium e hlaha le libaka tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le lithethefatsi tse kang katG 315G>C\A, InhA-15C>T, liphetho tsa liteko li bontša hore ha ho na khanyetso ho rifampicin, ho bolelang hore ha ho na cross-reactivity.
Lisebelisoa tse Sebetsang:	SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd. )