Influenza A/B
Lebitso la sehlahisoa
HWTS-RT003A Influenza A/B Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kokoana-hloko ea Influenza A ke lefu le tšoaetsanoang le matla la ho hema, le nang le mefuta e mengata e kang H1N1 le H3N2, e atisang ho fetoha 'me e hlahile lefatšeng ka bophara.Phetoho ea antigenic e bolela phetoho ea kokoana-hloko ea ntaramane A, e bakang ho hlaha ha mofuta o mocha.Livaerase tsa mokakallane oa B li arotsoe ka meloko e 'meli e meholo, Yamagata le Victoria.Livaerase tsa Influenza B li na le ho hoholeha ha antigenic feela, 'me li qoba ho lebela le ho hlakoloa ha sesole sa 'mele oa motho ka liphetoho tsa tsona.Leha ho le joalo, tekanyo ea ho iphetola ha lintho ea kokoana-hloko ea ntaramane ea B e lieha ho feta ea kokoana-hloko ea ntaramane A, 'me kokoana-hloko ea ntaramane ea B e ka boela ea baka tšoaetso ea matšoafo a batho 'me ea lebisa ho mafu a seoa.
Channel
| FAM | IFV A |
| ROX | Taolo ea ka hare |
| VIC/HEX | IFV B |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | sekoahelo sa oropharyngeal |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A:500Copies/mL,IFV B:500Copies/mL |
| Tse khethehileng | 1. Cross-reactivity: ha ho na cross reactivity between this kit le adenovirus type 3, 7, human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, and HCoV-NL63, cytomegalovirus, enterovirus, kokoana-hloko ea parainfluenza, kokoana-hloko ea maselese, metapneumovirus ea motho, kokoana-hloko ea mumps, ho hema syncytial kokoana-hloko ea mofuta oa B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraraxella catarrhalis pneumoniae mycoplasmosis, mycobacillus, moralla catarrhalis, pneumoniae, mycoplasmosis tidis , neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius le DNA ea motho ea genomic. 2. Teko ea tšitiso: Lintho tse sitisang li khethoa e le mucin (60mg/mL), mali a motho, oxymetazoline (2mg/mL), sebabole (10%), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir phosphate (60ng/mL), ribavirin (10mg/L), ’me liphetho li bontša hore lintho tse kena-kenanang le likhakanyo tse ka holimo ha li na karabelo e sitisang ho fumanoa ha kit. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Nako PCR System le BioRad CFX Opus 96 Real-Time PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Li-reagents tse khothalelitsoeng: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ho latela IFU.Molumo oa mohlala oa ho ntšoa ke200μL.Molumo o khothaletsoang oa elution ke 80μL.
Khetho ea 2.
Li-reagents tse khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ho ntšoa ho lokela ho etsoa ho latela IFU.
Khetho ea 3.
Li-reagents tse khothalelitsoeng: Nucleic Acid Extraction kapa Purification Kit (YDP315-R).Ho ntšoa ho lokela ho etsoa ho latela IFU.Bophahamo ba sampole ea ho ntšoa ke 140μL.Molumo o khothaletsoang oa elution ke 60μL.
