Mefuta e 14 ea Kotsi e Phahameng ea Papillomavirus ea Motho (16/18/52 Typing)
Lebitso la sehlahisoa
HWTS-CC019A-Mefuta e 14 ea Kotsi e Phahameng ea Papillomavirus ea Motho (16/18/52 Typing) Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Liphuputso li bontšitse hore tšoaetso ea HPV e phehellang le mafu a mangata ke e 'ngoe ea lisosa tse ka sehloohong tsa mofetše oa molomo oa popelo.Hajoale, liphekolo tse lemohuoeng tse sebetsang hantle li ntse li haella bakeng sa mofetše oa popelo o bakoang ke HPV, kahoo ho sibolloa kapele le thibelo ea tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela mofetše oa popelo.Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea bongaka le kalafo ea mofetše oa popelo.
Channel
| Channel | Mofuta |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Quasar 705/CY5.5 | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Moroto, Popelo, Swab ea Bosali |
| Ct | ≤28 |
| LoD | 300 likopi / mL |
| Tse khethehileng | Ha ho cross-reactivity le lisampole tse ling tsa phefumoloho tse kang Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, kokoana-hloko ea Coxsackie, kokoana-hloko ea Echo, Metapneuvirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, joalo-joalo le DNA ea genomic ea motho. |
| Lisebelisoa tse sebetsang | MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Nako PCR System le BioRad CFX Opus 96 Real-Time PCR System |
Phallo ea Mosebetsi
1.Mohlala oa moroto
A: Nka1.4mL ea sampole ea moroto e tla hlahlojoa le centrifuge ho 12000rpm metsotso e 5;lahla supernatant (ho kgothaletswa ho boloka 10-20μL supernatant ho tloha botlaaseng ba tube ea centrifuge), eketsa 200μL ea reagent ea ho lokolla sampole, 'me ho ntšoa ho latelang ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea Macro & Micro-Test Sample Release. Reagent (HWTS-3005-8).
B: Nka1.4mL ea sampole ea moroto e tla hlahlojoa le centrifuge ho 12,000rpm metsotso e 5;lahla matla a phahametseng tlhaho (ho kgothaletswa ho boloka 10-20μL ea supernatant ho tloha tlase ea centrifuge tube), 'me u kenye 200μL ea saline e tloaelehileng ho emisa hape, e le sampuli e lokelang ho lekoa.Tlhahiso e latelang e ka etsoa ka Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ho latela litaelo ka tieos bakeng sa tshebediso.Molumo o khothaletsoang oa elution ke 80μL.
C: Nka1.4mL ea sampole ea moroto e tla hlahlojoa le centrifuge ho 12,000rpm metsotso e 5;lahla matla a phahametseng tlhaho (ho kgothaletswa ho boloka 10-20μL ea supernatant ho tloha tlase ea centrifuge tube), 'me u kenye 200μL ea saline e tloaelehileng ho emisa hape, e le sampuli e lokelang ho lekoa.Ho ntšoa ho latelang ho ka etsoa kaQIAamp DNA Mini Kit(51304) ka QIAGEN kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ho ntšoa ho lokela ho sebetsoa ho latela litaelo tsa tšebeliso.Bophahamo ba sampole ea tlhahiso ke 200μL, 'me molumo o khothaletsoang oa tlhahiso ke 80μL.
2. Sesepa sa molomo oa popelo/mohlala
A: Nka 1mL ea sampole ho lekoa ka 1.5mLof tube ea centrifuge,lecentrifuge ka 12000rpm bakeng sa metsotso e 5. Discard the supernatant (ho kgothaletswa ho boloka 10-20μL ea supernatant ho tloha tlase ho tube ea centrifuge), eketsa 100μL ea reagent ea ho lokolla sampole, ebe o ntša ho latela litaelo tsa tšebeliso ea Macro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Ho ntšoa ho ka etsoa ka Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ho latela litaelo tse thata tsa tšebeliso.Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 80μL.
C: Ho ntšoa ho ka etsoa ka QIAamp DNA Mini Kit(51304) ka QIAGEN kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ho ntšoa ho lokela ho sebetsoa ho latela litaelo tsa tšebeliso.Bolumo ea sampole ea ho ntšoa ke 200 μL, 'me bophahamo ba molumo o khothaletsoang ke80 μL.
3, Mokokotlo oa Popelo/Mokhoa oa Bosali
Pele o etsa sampole, sebelisa swab ea k'hothone ho hlakola li-secretions tse feteletseng molomong oa popelo, 'me u sebelise sesepa e' ngoe ea k'hothone e kentsoeng ka tharollo ea sele kapa "cervical exfoliated cell sampling brush" ho itšoarella ka har'a molomo oa popelo 'me u fetole li-clockwise tse 3-5 ho fumana. cervical exfoliated cell.Butle-butle ntša swab ea k'hothone kapa borashe,lee kenye ka har'a mohlala oa tube e nang le 1mL ea letsoai le tloaelehileng le hloekisitsoeng. AHa u qeta ho hlatsoa ka ho feletseng, peta sesepa sa k'hothone se omeletseng kapa borashe khahlanong le lebota la tube 'me u lahle, tiisa sekoahelo sa tube, 'me u tšoaee mohlala oa lebitso (kapa nomoro) ebe u thaepa pompong ea mohlala.
