Likokoana-hloko tse Phefumolohang li Kopanetsoe

Likokoana-hloko tse Phefumolohang tse Kopantsoeng Setšoantšo se Featured

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba likokoana-hloko tsa ho hema ka nucleic acid e nkiloeng ho batho ba oropharyngeal swab.Likokoana-hloko tse fumanoeng li kenyelletsa: kokoana-hloko ea ntaramane A (H1N1, H3N2, H5N1, H7N9), kokoana-hloko ea ntaramane B (Yamataga, Victoria), kokoana-hloko ea parainfluenza (PIV1, PIV2, PIV3), metapneumovirus (A, B), adenovirus (1, 2, 3). , 4, 5, 7, 55), ho hema syncytial (A, B) le kokoana-hloko ea maselese.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-RT106A-Likokoana-hloko tsa Phefumoloho tse Kopantsoeng tsa ho lemoha (Fluorescence PCR)

Epidemiology

Mafu a bakoang ke likokoana-hloko tsa pathogenic tse hlaselang lesapo la nko la motho, 'metso, trachea, bronchus, matšoafo le lisele tse ling tsa ho hema le litho tsa' mele le ho ikatisa li bitsoa tšoaetso ea matšoafo.Liponahatso tsa bongaka li kenyelletsa feberu, ho khohlela, nko e tsoang, 'metso o bohloko, mokhathala o akaretsang le ho opeloa .Likokoana-hloko tsa ho hema li kenyelletsa likokoana-hloko, mycoplasma, chlamydia, libaktheria, joalo-joalo. Boholo ba tšoaetso bo bakoa ke likokoana-hloko.Likokoana-hloko tsa phefumoloho li na le litlhaku tse latelang tse kang mefuta e mengata ea mefuta, ho iphetola ha lintho ka potlako, li-subtypes tse rarahaneng, matšoao a tšoanang a kliniki.E na le litlhaku tsa kliniki tse kang ho qala ka potlako, ho ata ka potlako, tšoaetso e matla, le matšoao a tšoanang ao ho leng thata ho a khetholla, a bakang tšokelo e tebileng bophelong ba batho.

Channel

FAM	IFV A, IFV B Victoria, PIV mofuta 1, hMPV mofuta 2, ADV, RSV mofuta A, MV·
VIC(HEX)	IFV B, H1, IFV B Yamagata, Referense ea ka hare
CY5	Tšebeliso ea ka hare, mofuta oa PIV 3, hMPV type1, RSV mofuta oa B
ROX	Referense ea ka hare, H3, PIV mofuta oa 2

Tekheniki Parameters

Polokelo	Mokelikeli: ≤-18℃ Lefifing
Shelf-bophelo	Likhoeli tse 9
Mofuta oa Mohlala	Li-swabs tsa oropharyngeal tse sa tsoa bokelloa
Ct	≤38
CV	≤5.0%
LoD	500Likopi / mL
Tse khethehileng	Ha ho na cross-reactivity le genome ea motho le likokoana-hloko tse ling tsa phefumoloho.
Lisebelisoa tse sebetsang	E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng. Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System