Middle East Respiratory Syndrome Coronavirus Nucleic Acid

Tlhaloso e Khutšoanyane:

Kit e sebelisetsoa ho lemoha boleng ba MERS coronavirus nucleic acid ka har'a nasopharyngeal swabs e nang le coronavirus ea Middle East Respiratory Syndrome (MERS).


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

Epidemiology

Middle East Respiratory Syndrome coronavirus (MERS-CoV), β-coronavirus e bakanglefu la phefumoloho ho batho, le ile la tsejoa ka lekhetlo la pele ho mokuli e motona ea lilemo li 60 ea shoeleng oa Saudi Arabia ka 24th July, 2012. Tlhahiso ea meriana ea tšoaetso ea MERS-CoV e tloha ho boemo bo sa tsitsang kapa matšoao a bonolo a ho hema ho ea ho lefu le matla la ho hema esita le lefu.

Channel

FAM RNA kokoana-hloko ea MERS
VIC(HEX)

Taolo ea ka hare

Tekheniki Parameters

Polokelo

≤-18℃ Lefifing

Shelf-bophelo

Likhoeli tse 9

Mofuta oa Mohlala

Li-swabs tsa nasopharyngeal tse sa tsoa bokelloa

CV

≤5.0%

Ct

≤38

LoD

1000 Likopi / mL

Tse khethehileng

Ha ho na cross-reactivity with human coronaviruses human coronavirus SARSr-CoV le likokoana-hloko tse ling tse tloaelehileng.

Lisebelisoa tse Sebetsang:

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Nako ea 'Nete ea PCR Systems

Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P

Sesebelisoa sa PCR sa LightCycler®480 Real-Time

LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi

MA-6000 Real-Nako Quantitative Thermal Cycler

Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96

BioRad CFX Opus 96 Real-Nako PCR System

Phallo ea Mosebetsi

Khetho ea 1.

Sehlahisoa se khothalelitsoeng: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) by Tiangen Biotech (Beijing) Co., Ltd.

Khetho ea 2.

Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


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