Vaerase ea Ebola ea Zaire
Lebitso la sehlahisoa
HWTS-FE008 Kit ea ho Fumana Kokwana-hloko ea Ebola ea Zaire (Fluorescence PCR)
Thuto ea mafu a seoa
Vaerase ea Ebola ke ea Filoviridae, e leng vaerase ea RNA e se nang likarolo tse peli e nang le khoele e le 'ngoe e se nang likarolo tse peli. Livaerase ke likhoele tse telele tse bolelele ba virion bo tloaelehileng ba 1000nm le bophara ba hoo e ka bang 100nm. Jenome ea vaerase ea Ebola ke RNA e se nang likarolo tse peli e nang le boholo ba 18.9kb, e nang le liprotheine tse 7 tsa sebopeho le protheine e le 'ngoe e se nang sebopeho. Vaerase ea Ebola e ka aroloa ka mefuta e kang Zaire, Sudan, Bundibugyo, Tai Forest le Reston. Har'a tsona, mofuta oa Zaire le mofuta oa Sudan li tlalehiloe li baka mafu a batho ba bangata ka lebaka la tšoaetso. EHF (Ebola Hemorrhagic Fever) ke lefu le tšoaetsanoang le bakoang ke vaerase ea Ebola. Batho ba tšoaetsoa haholo-holo ke ho kopana le maro a 'mele, mantle le mantle a bakuli kapa liphoofolo tse tšoaelitsoeng, 'me matšoao a kliniki ke feberu e hlahellang, ho tsoa mali le tšenyo e ngata ea litho. EHF e na le sekhahla se phahameng sa lefu sa 50%-90%.
Mokero
| FAM | Asiti ea nucleic ea MP |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Serum e ncha, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 500/μL |
| Ho hlaka | Sebelisa lisebelisoa ho leka litšupiso tse mpe tsa k'hamphani, liphetho li fihlela litlhoko. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete, le Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX Opus 96 |
Phallo ea Mosebetsi
Khetho ea 1.
Reagent e kgothalletswang ho ntsha: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction kapa Purification Reagent (YDP315-R) ke Tiangen Biotech (Beijing) Co., Ltd. E lokela ho ntshwa ho ya ka ditaelo, mme bophahamo bo kgothalletswang ba ho ntsha sampole ke 140μL mme bophahamo bo kgothalletswang ba elution ke 60μL.
Khetho ea 2.
Reagent e kgothalletswang ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). E lokela ho ntshwa ho ya ka ditaelo. Bophahamo ba sampole ya ho ntsha ke 200μL, mme bophahamo bo kgothaletswang ba elution ke 80μL.
Khetho ea 3.
Li-reagent tse khothaletsoang tsa ho ntša: Setlolo sa Nucleic Acid Extraction Reagent (1000020261) le High Throughput Automated Sample Preparation System (MGISP-960) ka BGI li lokela ho ntšoa ho latela litaelo. Bophahamo ba ho ntša ke 160μL, 'me bophahamo ba elution bo khothaletsoang ke 60μL.
