Zaire Ebola Virus
Lebitso la Product
Hwts-fe008 zaire vashala vicleic acid kit (fluorescence pcr)
Epidphiology
Virus ea Ebola ke ea Filoviridae, eo e leng vaerase e sa reroang e sa reroang e sa reroang. Likoloi ke likhechana tse telele ka bolelele ba 'mala oa bo-bong ba 1000nm le bophara ba kabo ea 100nm. Genole vaeba ea vaerase ke ea li-strand tse sa reroang tse sa lokang Virus ea Ebola e ka aroloa ka mefuta e kang Zaiire, Skudan, Budan, Tai Tai le Reston. Har'a bona, mofuta oa Zaire le mofuta oa Sudan o tlalehiloe hore a bolaea batho ba bangata ba tšoaeang tšoaetso. Ehf (Ebola hemorrhagic fever) Batho ba tšoaelitsoe haholo-holo ka maro a 'mele, liphiri le liphoofolo tse tšoaelitsoeng ke bakuli kapa lipontšo tsa bongaka hangata li senyeha. Ehf e na le sekhahla se phahameng sa lefu la 50% -90%.
Channel
| FMAM | Mp Nucleic Acid |
| Rox | Taolo e ka hare |
Litekanyetso tsa tekheniki
| Polokelo | ≤-18 ℃ |
| Shell-Bophelo | Likhoeli tse 9 |
| Mofuta oa SIMON | Serum se secha serum, plasma |
| Tt | ≤38 |
| CV | ≤ 40.0% |
| Lod | 500 likopi / μl |
| TLHOKOMELISO | Sebelisa kits ho lekola litšupiso tse mpe tsa k'hamphani, liphetho li fihlela litlhokahalo. |
| Lisebelisoa tse sebetsang | E sebelisoa BIOSSYSTEMS 7500 Real-Nako ea Pruc Biosstems ea BiosStems 7500 ea Nako e sa Ts'oerehileng ea Nako ea Nako ea Nako Quantshetstudio®5 Real-Nako ea Likarolo Slan-96p Real-Nako ea 'nete ea £ Hongbili Medical Lightcycler®480 Real-Nako ea PCR ea Pru Linegene 9600 PLs Sistimi ea Nako ea Tlhaho (Fqd-96a, bioer ea Hangzhou Ma-6000 Real-Nako ea nnete ea Thermal cycler (Suzhou Molayy Co, Ltd.) Biorad CFX96 Real-Nako ea Pru ea Prusi, 'me biorad Cfx op 96 |
Phallo ea mosebetsi
Khetho ea 1.
E khothalelitsoeng ea ho itlosa bolutu: qiaamp viral rna mini kit (52904), Nucleic Acien (Beijing) Co, E ne e lokela ho ntšoa maemong a tiileng ho litaelo, 'me bophahamo ba mohlala o khothalelitsoeng ke 140μl le Mobu o khothalelitsoeng ke 60μl.
Khetho ea 2.
Khokahano e ntlafalitsoeng ea ho itlosa bolutu: macro le micro-te Viral Dna / Rna Kit (HWTS-300-94-96) le Micro-3006) .it e lokela ho nkuoa ho latela litaelo. Molumo oa mohlala oa ho tlosa molumo oa molumo oa 1 o 200μl, 'me molumo oa phatlalatso o khothalelitsoeng o 80μl.
Khetho ea 3.
Rekadbonts e khothalelitsoeng: Thekofatso ea Nucleic acid Buka ea tlhahiso ea 160μl, 'me Buka e khothalelitsoeng ea ho lumelisa e na le 60μl.
