Enterococcus e hananang le Vancomycin le Gene e hananang le meriana
Lebitso la sehlahisoa
HWTS-OT090-Enterococcus e hananang le Vancomycin le Kit ea ho Fumana Liphatsa tsa Lefutso e hananang le Meriana (Fluorescence PCR)
Thuto ea mafu a seoa
Ho hanyetsa lithethefatsi ho boetse ho tsejoa e le ho hanyetsa lithethefatsi, ho bolela ho hanyetsa ha libaktheria ketsong ea meriana e loantšang likokoana-hloko. Hang ha ho hanyetsa lithethefatsi ho hlaha, phello ea meriana ea chemotherapy e tla fokotseha haholo. Ho hanyetsa lithethefatsi ho arotsoe ka ho hanyetsa ka hare le ho hanyetsa ho fumanoeng. Ho hanyetsa ka hare ho khethoa ke liphatsa tsa lefutso tsa baktheria, tse fetisetsoang ho tloha molokong o mong ho ea ho o mong, 'me li ke ke tsa fetoha. Ho hanyetsa ho fumanoeng ho bakoa ke taba ea hore kamora ho kopana le lithibela-mafu, libaktheria li fetola litsela tsa tsona tsa metabolism e le hore li se ke tsa bolaoa ke lithibela-mafu.
Liphatsa tsa lefutso tsa ho hanyetsa vancomycin VanA le VanB ke ho hanyetsa meriana ho fumanoeng, tseo har'a tsona VanA e bonts'ang maemo a holimo a ho hanyetsa vancomycin le teicoplanin, VanB e bonts'a maemo a fapaneng a ho hanyetsa vancomycin, 'me e na le kutloelo-bohloko ho teicoplanin. Vancomycin hangata e sebelisoa ka bongaka ho phekola tšoaetso ea baktheria ea Gram-positive, empa ka lebaka la ho hlaha ha enterococci e hanyetsanang le vancomycin (VRE), haholo-holo enterococcus faecalis le enterococcus faecium, tse etsang karolo e fetang 90%, e tlisitse liphephetso tse ncha tse kholo kalafong ea bongaka. Hona joale, ha ho na moriana o itseng oa antibacterial bakeng sa kalafo ea VRE. Ho feta moo, VRE e ka boela ea fetisetsa liphatsa tsa lefutso tsa ho hanyetsa meriana ho enterococci e 'ngoe kapa libaktheria tse ling tsa Gram-positive.
Mokero
| FAM | Enterococci e hananang le Vancomycin (VRE): Enterococcus faecalis le Enterococcus faecium |
| VIC/HEX | Taolo ea Ka Hare |
| CY5 | Jini ea ho hanyetsa vancomycin VanB |
| ROX | Jini ea ho hanyetsa vancomycin VanA |
Li-parameter tsa Tekheniki
| Polokelo | Metsi: ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | sekhohlela, mali, moroto kapa likolone tse hloekileng |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| Ho hlaka | Ha ho na karabelo e kopanang le dikokwana-hloko tse ding tsa ho hema tse kang klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemolyticus, legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, kapa disampole di na le dijini tse ding tse hananang le meriana CTX, mecA, SME, Disampole tsa SHV le TEM. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Li-reagent tse khothaletsoang tsa ho ntša: Kit ea DNA ea Genomic ea Macro le Micro-Test (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) le Macro le Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
