Likokoana-hloko tse Phefumolohang li Kopanetsoe
Lebitso la sehlahisoa
HWTS-RT158A Likokoana-hloko tse Phefumolohang tse Kopantsoeng tsa Temoho(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Lefu la Corona Virus 2019, le bitsoang'COVID 19', e bua ka pneumonia e bakoang ke ts'oaetso ea 2019-nCoV.2019-nCoV ke coronavirus ea mofuta oa β.COVID-19 ke lefu le tšoaetsanoang le matla la phefumoloho, 'me baahi ba bangata hangata ba kotsing.Hajoale, mohloli oa tšoaetso ke bakuli ba tšoaelitsoeng haholo ke 2019-nCoV, mme batho ba tšoaelitsoeng ba asymptomatic le bona e ka ba mohloli oa ts'oaetso.Ho ipapisitsoe le lipatlisiso tsa morao-rao tsa epidemiological, nako ea ho incubation ke matsatsi a 1-14, haholo matsatsi a 3-7.Feberu, ho khohlela ho omileng le mokhathala ke lipontšo tse ka sehloohong.Bakuli ba seng bakae ba ne ba e-na le matšoao a kang ho tsubella nko, nko e phallang, 'metso o bohloko, myalgia le letšollo, joalo-joalo.
Influenza, eo hangata e tsejoang e le "ntaramane", ke lefu le tšoaetsanoang le matla la ho hema le bakoang ke vaerase ea ntaramane.E tšoaetsanoa haholo.E fetisoa haholo ke ho khohlela le ho thimola.Hangata e hlaha nakong ea selemo le mariha.Likokoana-hloko tsa ntaramane li arotsoe ka ntaramane A (IFV A), ntaramane B (IFV B), le Influenza C (IFV C) mefuta e meraro, kaofela ke ea kokoana e khomarelang, e bakang lefu la batho haholo-holo bakeng sa likokoana-hloko tsa ntaramane A le B, ke mofuta o le mong. kokoana-hloko ea RNA e arohaneng, e arohaneng.Kokoana-hloko ea Influenza A ke ts'oaetso e matla ea ho hema, e kenyeletsang H1N1, H3N2 le mefuta e meng e nyenyane, e atisang ho fetoha le ho qhoma lefatšeng ka bophara."Shift" e bolela phetoho ea kokoana-hloko ea ntaramane A, e hlahisang ho hlaha ha kokoana-hloko e ncha "subtype".Livaerase tsa mokakallane oa B li arotsoe ka mela e 'meli, Yamagata le Victoria.Kokoana-hloko ea Influenza B e na le ho hoholeha ha antijeni, 'me e qoba ho lekoa le ho felisoa ha sesole sa 'mele oa motho ka phetoho ea eona.Leha ho le joalo, lebelo la ho iphetola ha lintho la kokoana-hloko ea ntaramane ea B le butle ho feta ba kokoana-hloko ea ntaramane A.Kokoana-hloko ea Influenza B e ka boela ea baka mafu a matšoafo a batho 'me ea baka mafu a seoa.
Respiratory syncytial virus (RSV) ke kokoana-hloko ea RNA, ea lelapa la paramyxoviridae.E fetisoa ke marotholi a moea le ho kopana haufi-ufi 'me ke eona kokoana-hloko e ka sehloohong ea tšoaetso e tlaase ea matšoafo ho masea.Masea a tšoaelitsoeng ke RSV a ka ’na a ba le bronchiolitis e matla le pneumonia, tse amanang le asma ho bana.Masea a na le matšoao a matla, ho kenyelletsa feberu e phahameng, rhinitis, pharyngitis le laryngitis, ebe joale la bronchiolitis le pneumonia.Bana ba seng bakae ba kulang ba ka ba thata le otitis media, pleurisy le myocarditis, joalo-joalo Tšoaetso ea phefumoloho e ka holimo ke letšoao le ka sehloohong la tšoaetso ho batho ba baholo le bana ba baholo.
Channel
| FAM | SARS-CoV-2 |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| Quasar 705 | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mL Kokoana-hloko ea Influenza A/Influenza B/virus ea ho hema ea syncytial: 500Copies/mL |
| Tse khethehileng | a) Liphetho tsa li-cross-reactivity li bontša hore ha ho na karabelo e fapaneng lipakeng tsa kit le coronavirus ea motho SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus ea motho, enterovirus A, B, C, D, kokoana ea epstein-barr, kokoana-hloko ea maselese, kokoana-hloko ea cytomegalo, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, mosi aspergillus, candida albicans, candida glabrata, pneumocystis pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, mosi aspergillus, candida albicans, candida glabrata, pneumocystis pyogenes ea batho ba bacha le cryptococcus jiroveccus ea batho. b) Bokhoni ba ho thibela tšitiso: khetha mucin (60mg/mL), 10% (v/v) ea mali le phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (ho kenyeletsoa li-preservatives) (20mg/mL) , beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL) , histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) le tobramycin (0.6mg/mL) ) bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bontša hore ho kena-kenana le lintho tse nang le likhahla tse boletsoeng ka holimo ha ho na ho kena-kenana le liphello tsa liteko tsa likokoana-hloko. |
| Lisebelisoa tse sebetsang | Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 Rotor-Gene Q 5plex HRM Platform ea nako ea sebele ea PCR System |
