Likokoana-hloko tse Phefumolohang li Kopanetsoe
Lebitso la sehlahisoa
HWTS-RT158A Likokoana-hloko tse Phefumolohang tse Kopantsoeng tsa Temoho(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Lefu la Corona Virus 2019, le bitsoang'COVID 19', e bua ka pneumonia e bakoang ke ts'oaetso ea 2019-nCoV. 2019-nCoV ke coronavirus ea mofuta oa β. COVID-19 ke lefu le tšoaetsanoang le matla la phefumoloho, 'me baahi ba bangata hangata ba kotsing. Hajoale, mohloli oa tšoaetso ke bakuli ba tšoaelitsoeng haholo ke 2019-nCoV, mme batho ba tšoaelitsoeng ba asymptomatic le bona e ka ba mohloli oa ts'oaetso. Ho ipapisitsoe le lipatlisiso tsa morao-rao tsa epidemiological, nako ea ho incubation ke matsatsi a 1-14, haholo matsatsi a 3-7. Feberu, ho khohlela ho omileng le mokhathala ke lipontšo tse ka sehloohong. Bakuli ba seng bakae ba ne ba e-na le matšoao a kang ho tsubella nko, nko e phallang, 'metso o bohloko, myalgia le letšollo, joalo-joalo.
Influenza, eo hangata e tsejoang e le "ntaramane", ke lefu le tšoaetsanoang le matla la ho hema le bakoang ke vaerase ea ntaramane. E tšoaetsanoa haholo. E fetisoa haholo ke ho khohlela le ho thimola. Hangata e hlaha nakong ea selemo le mariha. Likokoana-hloko tsa ntaramane li arotsoe ka ntaramane A (IFV A), ntaramane B (IFV B), le Influenza C (IFV C) mefuta e meraro, kaofela ke ea kokoana-hloko e khomarelang, e bakang lefu la batho haholo-holo bakeng sa likokoana-hloko tsa ntaramane A le B, ke kokoana-hloko e le 'ngoe, e arohaneng ea RNA. Kokoana-hloko ea Influenza A ke ts'oaetso e matla ea ho hema, e kenyeletsang H1N1, H3N2 le mefuta e meng e nyenyane, e atisang ho fetoha le ho qhoma lefatšeng ka bophara. "Shift" e bolela phetoho ea kokoana-hloko ea ntaramane A, e hlahisang ho hlaha ha kokoana-hloko e ncha "subtype". Livaerase tsa mokakallane oa B li arotsoe ka mela e 'meli, Yamagata le Victoria. Kokoana-hloko ea Influenza B e na le ho hoholeha ha antijeni, 'me e qoba ho lekoa le ho felisoa ha sesole sa 'mele oa motho ka phetoho ea eona. Leha ho le joalo, lebelo la ho iphetola ha lintho la kokoana-hloko ea ntaramane ea B le butle ho feta ba kokoana-hloko ea ntaramane A. Kokoana-hloko ea Influenza B e ka boela ea baka mafu a matšoafo a batho 'me ea baka mafu a seoa.
Respiratory syncytial virus (RSV) ke kokoana-hloko ea RNA, ea lelapa la paramyxoviridae. E fetisoa ka marotholi a moea le ho kopana haufi-ufi 'me ke eona kokoana-hloko e ka sehloohong ea tšoaetso e tlaase ea matšoafo ho masea. Masea a tšoaelitsoeng ke RSV a ka ’na a ba le bronchiolitis e matla le pneumonia, tse amanang le asma ho bana. Masea a na le matšoao a matla, ho kenyelletsa feberu e phahameng, rhinitis, pharyngitis le laryngitis, ebe joale la bronchiolitis le pneumonia. Bana ba seng bakae ba kulang ba ka ba thata le otitis media, pleurisy le myocarditis, joalo-joalo Tšoaetso ea phefumoloho e ka holimo ke letšoao le ka sehloohong la tšoaetso ho batho ba baholo le bana ba baholo.
Channel
| FAM | SARS-CoV-2 |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| Quasar 705 | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mL Kokoana-hloko ea Influenza A/Kokoana-hloko ea Mofoka B/Vaerase ea ho hema ea syncytial: 500Copies/mL |
| Tse khethehileng | a) Liphetho tsa ts'ebetso e fapaneng li bonts'a hore ha ho na karabelo e fapaneng lipakeng tsa kit le coronavirus ea motho SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, human pneumoniae, pneumoniae, C, pneumoniae, C, C A, B, C, D, kokoana-hloko ea epstein-barr, kokoana-hloko ea maselese, kokoana-hloko ea cytomegalo, rotavirus, norovirus, kokoana-hloko ea parotitis, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniaecus pneumogenesis, myogenesis lefuba, mosi oa aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci le cryptococcus e sa tsoa tsoaloa le human genomic nucleic acid. b) Bokhoni ba ho thibela ho kena-kenana: khetha mucin (60mg/mL), 10% (v/v) ea mali le phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (ho kenyeletsoa li-preservatives) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), dexamenisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamimvirim/mL), zanamimvirim/mL (10ba rimg rimg), osenamivir (20) (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxamy/mL), levofloxamy (0.6mg / mL) bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bontša hore ho kena-kenana le lintho tse nang le likhahla tse boletsoeng ka holimo ha ho na ho kena-kenana le liphello tsa liteko tsa likokoana-hloko. |
| Lisebelisoa tse sebetsang | Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 Rotor-Gene Q 5plex HRM Platform ea nako ea sebele ea PCR System |
