Orientia tsutsugamushi
Lebitso la sehlahisoa
HWTS-OT002B Orientia tsutsugamushiNucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Scrub typhus ke lefu le matla la febrile le bakoang ke tšoaetso ea Orientia tsutsugamushi (Ot). Orientia scrub typhus ke gram-negative obligate intracellular parasitic microorganism. Orientia scrub typhus ke ea mofuta oa Orientia ka tatellano ea Rickettsiales, lelapa la Rickettsiaceae, le mofuta oa Orientia. Scrub typhus e fetisoa haholo-holo ka ho longoa ke li-chigger larvae tse jereng likokoana-hloko. E tšoauoa ka tleliniking ka tšohanyetso feberu e phahameng, eschar, lymphadenopathy, hepatosplenomegaly, le peripheral mali leukopenia, joalo-joalo Maemong a matla, ho ka etsa hore meningitis, sebete le liphio ho hloleha, systemic tse ngata tsa setho ho hloleha, esita le lefu.
Channel
| FAM | Orientia tsutsugamushi |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | serum e ncha |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection Systems (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Nako PCR System, BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng sa ho ntša: Macro & Micro-TestKakaretsoDNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ho ea ka taelo ea tšebeliso ea reagent ena ea ho ntša. Bolumo ea sampole e ntšitsoeng ke 200µL, 'me bophahamo ba molumo o khothaletsoang ke100µL.
