Orientia tsutsugamushi
Lebitso la sehlahisoa
HWTS-OT002B Orientia tsutsugamushiKiti ea ho Fumana Asiti ea Nyutlelie (Fluorescence PCR)
Thuto ea mafu a seoa
Scrub typhus ke lefu le matla la feberu le bakoang ke tšoaetso ea Orientia tsutsugamushi (Ot). Orientia scrub typhus ke likokoana-hloko tse kenang ka hare ho sele tse nang le Gram-negative. Orientia scrub typhus ke ea mofuta oa Orientia ka tatellano ea Rickettsiales, lelapa la Rickettsiaceae, le mofuta oa Orientia. Scrub typhus e fetisoa haholo-holo ka ho longoa ke liboko tsa chigger tse jereng likokoana-hloko. E khetholloa ka bongaka ka feberu e phahameng ea tšohanyetso, eschar, lymphadenopathy, hepatosplenomegaly, le peripheral blood leukopenia, jj. Maemong a boima, e ka baka meningitis, ho hloleha ha sebete le liphio, ho hloleha ha litho tse ngata tsa 'mele, esita le lefu.
Mokero
| FAM | Orientia tsutsugamushi |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | serum e foreshe |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 500/μL |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 LineGene 9600 Plus Mekhoa ea ho Lemoha PCR ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete, Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e khothaletsoang ea ho ntša: Macro le Micro-TestKakaretsoKiti ea DNA/RNA (HWTS-301)9) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso ea reagent ena ea ho ntšoa. Bophahamo ba sampole e ntšitsoeng ke 200µL, 'me bophahamo bo khothaletsoang ba elution ke100µL.
