Mycoplasma Pneumoniae (MP)
Lebitso la sehlahisoa
HWTS-RT024 Kit ea ho Fumana Sefuba sa Nyutlelie ea Mycoplasma Pneumoniae (MP) (Fluorescence PCR)
Thuto ea mafu a seoa
Mycoplasma pneumoniae (MP) ke mofuta oa likokoana-hloko tse nyane ka ho fetisisa tsa prokaryotic, tse pakeng tsa baktheria le vaerase, tse nang le sebopeho sa sele empa li se na lebota la sele. MP e baka tšoaetso ea phefumoloho ea batho haholo-holo ho bana le bacha. E ka baka pneumonia ea mycoplasma ea batho, tšoaetso ea phefumoloho ea bana le pneumonia e sa tloaelehang. Lipontšo tsa kliniki li fapane, tseo boholo ba tsona e leng khohlela e matla, feberu, serame, hlooho e opang, 'metso o bohloko. Tšoaetso ea phefumoloho e ka holimo le pneumonia ea bronchial ke tsona tse atileng haholo. Bakuli ba bang ba ka hlaha ho tloha tšoaetsong ea phefumoloho e ka holimo ho ea pneumonia e matla, ho hema ho matla le lefu le ka hlaha.
Mokero
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sekhohlela, swab ea oropharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 200/mL |
| Ho hlaka | a） Ho arabela ka tsela e fapaneng: ha ho na ho arabela ka tsela e fapaneng le Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Vaerase ea Influenza A, Vaerase ea Influenza B, Vaerase ea Parainfluenza mofuta oa I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Vaerase ea syncytial ea phefumoloho le human genomic nucleic acid. b）Bokhoni ba ho thibela tšitiso: ha ho na tšitiso ha lintho tse sitisang li lekoa ka maemo a latelang: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) mali a motho, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
(1) Sampole ea Sekhohlela
Reagent e kgothalletswang ya ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 200µL ya saline e tlwaelehileng ho precipitate e sebeditsweng. Ho ntsha ho latelang ho lokela ho etswa ho ya ka taelo ya tshebediso. Bophahamo bo kgothalletswang ba elution ke 80µL. Reagent e kgothalletswang ya ho ntsha: Nucleic Acid Extraction kapa Purification Reagent (YDP315-R). Ho ntsha ho lokela ho etswa ka thata ho ya ka taelo ya tshebediso. Bophahamo bo kgothalletswang ba elution ke 60µL.
(2) Sesepa sa molomo oa molomo
Reagent e kgothalletswang ya ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso. Bophahamo bo kgothalletswang ba ho ntsha sampole ke 200µL, mme bophahamo bo kgothalletswang ba elution ke 80µL. Reagent e kgothalletswang ya ho ntsha: QIAamp Viral RNA Mini Kit (52904) kapa Nucleic Acid Extraction kapa Purification Reagent (YDP315-R). Ho ntsha ho lokela ho etswa ka thata ho ya ka ditaelo tsa tshebediso. Bophahamo bo kgothaletswang ba ho ntsha sampole ke 140µL, mme bophahamo bo kgothaletswang ba ho ntsha sampole ke 60µL.
