Mycoplasma Pneumoniae (MP)
Lebitso la sehlahisoa
HWTS-RT024 Mycoplasma Pneumoniae (MP) ea Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) ke mofuta oa kokoana-hloko e nyenyane ka ho fetisisa ea prokaryotic, e pakeng tsa baktheria le kokoana-hloko, e nang le sebopeho sa lisele empa e se na lerako la sele. MP haholo e baka tšoaetso ea phefumoloho ea batho, haholo-holo ho bana le bacha. E ka baka pneumonia ea mycoplasma ea motho, tšoaetso ea matšoafo ea bana le pneumonia e sa tloaelehang. Lipontšo tsa kliniki li fapane, boholo ba tsona ke khohlela e matla, feberu, serame, hlooho e bohloko, 'metso. Tšoaetso e ka holimo ea matšoafo le pneumonia ea bronchial ke tsona tse atileng haholo. Bakuli ba bang ba ka hlaha ho tloha ho ts'oaetso e phahameng ea phefumoloho ho isa ho pneumonia e matla, khatello e matla ea ho hema le lefu le ka hlaha.
Channel
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sputum, Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 likopi / mL |
| Tse khethehileng | a） Cross reactivity: ha ho na cross reactivity with Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacteriudococcus tuberlasis, Pphilamonasis aeruginosa, Acinetobacter baumannii, kokoana-hloko ea Influenza A, kokoana-hloko ea Influenza B, Parainfluenza kokoana-hloko ea mofuta oa I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, kokoana-hloko ea Respiratory syncytial le human genomic nucleic acid. b) Bokhoni ba ho thibela tšitiso: ha ho na tšitiso ha lintho tse sitisang li ne li lekoa ka lintlha tse latelang: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) mali a motho, levofloxacin (10μg/mL), moxiflogxacin/mL), moxifloxacin/0. azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
(1) Mohlala oa sekhohlela
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid006W Extra HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 200µL ea letsoai le tloaelehileng ho pula e lokiselitsoeng. Ho ntšoa ka mor'a moo ho lokela ho etsoa ho latela litaelo tsa tšebeliso. Bophahamo bo khothaletsoang ba tlhahiso ke 80µL. Sehlahisoa se khothalelitsoeng sa ho hula: Nucleic Acid Extraction kapa Purification Reagent (YDP315-R). Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo tsa tšebeliso. Molumo o khothaletsoang oa elution ke 60µL.
(2) Oropharyngeal swab
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid006W Extra HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso. Bolumo e khothaletsoang ea ho ntšoa ea sampole ke 200µL, 'me bophahamo ba molumo o khothaletsoang ke 80µL. Recommended extraction reagent: QIAamp Viral RNA Mini Kit (52904) kapa Nucleic Acid Extraction or Purification Reagent (YDP315-R). Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo tsa tšebeliso. Molumo o khothaletsoang oa ho ntša sampole ke 140µL, 'me bophahamo ba modumo o khothaletsoang ke 60µL.
