Klebsiella Pneumoniae, Acinetobacter Baumannii le Pseudomonas Aeruginosa le Liphatsa tsa lefutso tsa ho hanyetsa lithethefatsi (KPC, NDM, OXA48 le IMP) Multiplex
Lebitso la sehlahisoa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii and Pseudomonas Aeruginosa and Drug Resistance Genes (KPC, NDM, OXA48 le IMP) Multiplex Detection Kit(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Klebsiella pneumoniae ke pathogen e tloaelehileng ea kliniki e nkang monyetla 'me ke e' ngoe ea libaktheria tsa bohlokoa tsa pathogenic tse bakang mafu a nosocomial.Ha khanyetso ea 'mele e fokotsehile, libaktheria li kena matšoafong ho tsoa ka pampiri ea ho hema, li baka tšoaetso likarolong tse ngata tsa' mele, 'me tšebeliso ea pele ea lithibela-mafu ke senotlolo sa ho phekola.[1].
Sebaka se atileng haholo sa ts'oaetso ea Acinetobacter baumannii ke matšoafo, e leng kokoana-hloko ea bohlokoa bakeng sa pneumonia e fumanoeng Sepetlele (HAP), haholo-holo Ventilator e amanang le pneumonia (VAP).Hangata e tsamaea le mafu a mang a baktheria le a fungal, a nang le litšobotsi tsa boemo bo phahameng ba ho kula le sekhahla se phahameng sa ho shoa.
Pseudomonas aeruginosa ke kokoana-hloko e sa belang ea gram-negative e tloaelehileng haholo ts'ebetsong ea bongaka, 'me ke kokoana-hloko ea bohlokoa e nkang monyetla bakeng sa tšoaetso e fumanoang sepetlele, e nang le litšobotsi tsa bokolone bo bonolo, ho fetoha habonolo le ho hanyetsa lithethefatsi tse ngata.
Channel
| Lebitso | PCR-Mix 1 | PCR-Mix 2 |
| Mokhatlo oa FAM | Aba | IMP |
| VIC/HEX Channel | Taolo ea ka hare | KPC |
| CY5 Channel | PA | NDM |
| ROX Channel | KPN | OXA48 |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sekhohlela |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/mL |
| Tse khethehileng | a) Teko ea cross-reactivity e bonts'a hore kit ena ha e na reactivity ea sefapano le likokoana-hloko tse ling tsa phefumoloho, joalo ka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter, Plefilamonolytics, Lefila, Lefila, Lefila, Lefila, Lefila, Epilamonas, Epilamonoli, Epilasa, Epilasa, Epilasa, Epilasa, Epilasa, Epilasa, Epilasa, Epilasa, Epilasa, Epilasa, Leago, Leago, Leago, Epilasa, Leifolau, Epila, Epila, Epila, Epilasa, Leago, Leago, Pylia, Epilamonola. fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus le disampole tsa sekhohlela ntle le liphofu, joalo-joalo. b) Bokhoni ba ho thibela tšitiso: Khetha mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, le roxithromycin, joalo-joalo bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bontša hore lintho tsa tšitiso tse boletsoeng ka holimo u se ke ua kena-kenana le ho fumanoa ha Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa le liphatsa tsa lefutso tsa carbapenem KPC, NDM, OXA48 le IMP. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Kakaretso ea Tharollo ea PCR
