Mycobacterium Tuberculosis Nucleic Acid le Rifampicin (RFF), Ho hanyetsa (Ithh)

Tlhaloso e khutšoane:

Kit ena e sebelisetsoa ho khetholla vitro ka mokhoa o hlakileng oa ambobacterium e le sputum ea motho, le liphetoho tse kholo tsa metsi , setereke se hanyetsanang se hanyetsanang sa ho khetha rifampicin e re: hammoho le liphetoho libakeng tse ka sehloohong tsa khokahano ea Mycobacterium, 'me e fana ka thuso ea ho hanyetsa harasiloko ea Mycobactereum Tuberculos e tšoaelitsoeng ke mokuli.


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HWTS-RT1BacGriasium

Epidphiology

Mycobactesium Tuberculosium, haufinyane joalo ka bactertures ea lefutso e bakang lefuba la lefu la lefu la lefutso le lefu la tuthogesion le ethampicolin, joalo-joalo[1]. Leha ho le joalo, ka lebaka la ts'ebeliso e fosahetseng ea lithethefatsi tsa lefutso tsa lefutso le litšobotsi tsa leboea la selekama sa mycobactereum, 'me ho bile le lefu le kotsi la lefuba (mdr- TB) e hanyetsa lithethefatsi tsena tse peli tse atileng ebile e sebetsang, Rifampicin le Isoniazid[2].

Bothata ba ho sebelisa lithethefatsi a lefubelu linaheng tsohle tse hlahisoang ke Who. Bakeng sa ho fana ka meralo e nepahetseng ea kalafo bakeng sa bakuli ba lefupelo, ho hlokahala hore ba hanyetsane lithethefatsi, e leng phetohelo ea lefu la lefutso e khothalelitsoeng[3]. Leha ho sibolloa ha rifampicin e batla e lekana le ho sibolla bakuli ka lebaka la rifampicin) empa e hana ho hanyetsa. rifampicin), e ka lebisang ho bakuli ba fuoang kalafo e sa utloahaleng ea pele LITLHAKISO. Ka hona, teko ea bohahlauli ba Isoniazid le Rifampic[4].

Litekanyetso tsa tekheniki

Polokelo

≤-18 ℃

Shell-Bophelo Likhoeli tse 12
Mofuta oa SIMON Sputum sampole ea sputum, setso se tiileng (LJ Medium), setso sa metsi (MGit Medium)
CV <5.0%
Lod Lod ea kititi bakeng sa ho lemoha tuberculosium tuberculosis ke li-bacteria / ml;Lod ea kit bakeng sa ho lemoha rifampicin mofuta oa mofuta oa mofuta o hlaha ke bacteria e 150 / ml;

Lod ea kit bakeng sa ho hopola mofuta oa sejalo sa Anonia o hlaha ke libaktheria / ml.

TLHOKOMELISO

1) Ha ho na karabelo ea sefapano ha u sebelisa Kit ho bona gechogents ea 4 ea lefutso (joalo ka ha ho bontšitsoe ho Lethathamo la 3).2) Ha ho na karabelo ea sefapano ha u sebelisa Kit ea ho kenyelletsa mefuta e meng ea lithethefatsi tse thibelang lithethefatsi tse ling tse tšelisang tsa rilicampiin le tafoleng ea 4).3) Lintho tse tloaelehileng tse tloaelehileng ka lasamples ho hlahlojoa, tse kang rifampicin (9mg / l), ethammetazelin (8mg / l), 1mg / l), 1mg / l), 1mg / l), 1mg / l), 1mg / l), 1mg / l) (20mg / L), pyrazinamide (45mg / l), Zanamivir (0.5mg / L), Dexamethasone (20mg / L), ha u na phello liphethong tsa liteko tsa kpa.
 Lisebelisoa tse sebetsang Slan-96p Real-Time Doer-Time Docr System (Hongfi: Theknoloji ea bongaka, Ltd.),

Biorad CFX96 Real-Nako ea Prust

Kakaretso ea Tharollo ea PCR


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