Mycobacterium Tuberculosis Nucleic Acid le Rifampicin(RIF)，Resistance(INH)
Lebitso la sehlahisoa
HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), (INH) Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, haufinyane joalo ka Tubercle bacillus (TB), ke baktheria ea pathogenic e bakang lefuba, 'me hajoale, litlhare tsa mofuta oa pele tse sebelisoang hangata tse thibelang lefuba li kenyelletsa isoniazid, rifampicin le ethambutol, joalo-joalo.[1]. Leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea lithethefatsi tse khahlanong le lefuba le litšoaneleho tsa sebopeho sa lerako la lisele tsa lefuba la mycobacterium ka boeona, lefuba la mycobacterium le hlahisitse ho hanyetsa lithethefatsi khahlanong le lefuba, 'me mofuta o kotsi haholo ke lefuba le hanyetsanang le lithethefatsi tse ngata (MDR-TB), le hanyetsanang le meriana e' meli e tloaelehileng le e sebetsang, rifampicin le eona.[2].
Bothata ba ho hanyetsa meriana ea lefuba bo teng linaheng tsohle tse entsoeng lipatlisiso ke WHO. E le ho fana ka merero e nepahetseng haholoanyane ea phekolo bakeng sa bakuli ba lefuba, hoa hlokahala ho lemoha khanyetso ho litlhare tse thibelang lefuba, haholo-holo rifampicin resistance, e fetohileng mohato oa tlhahlobo o khothaletsoang ke WHO kalafong ea lefuba.[3]. Le hoja ho sibolloa ha rifampicin ho hanyetsa ho batla ho lekana le ho sibolloa ha MDR-TB, ho lemoha feela khanyetso ea rifampicin ho hlokomoloha bakuli ba nang le mono-resistant INH (e bolelang ho hanyetsa isoniazid empa e le bosisi ho rifampicin) le mono-resistant rifampicin (kutloisiso ho isoniazid empa ho hanyetsa rifampicin), e leng se ka lebisang ho bakuli ba sa utloahaleng ho qala kalafo. Ka hona, liteko tsa isoniazid le rifampicin ke litlhokahalo tse hlokahalang haholo mananeong ohle a taolo ea DR-TB[4].
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Mohlala oa sekhohlela, Setso se Tiileng (LJ Medium), Setso sa Mokelikeli (MGIT Medium) |
| CV | <5.0% |
| LoD | LoD ea khiti bakeng sa ho lemoha lefuba la Mycobacterium ke libaktheria tse 10/mL;LOD ea khiti bakeng sa ho lemoha mofuta o hlaha oa rifampicin le mofuta o fetotsoeng ke 150 bacteria/mL; LOD ea khiti bakeng sa ho lemoha mofuta oa hlaha oa isoniazid le mofuta o fetohileng ke 200 bacteria/mL. |
| Tse khethehileng | 1) Ha ho na karabelo ea sefapano ha u sebelisa kit ho lemoha DNA ea motho ea genomic (500ng), mefuta e meng ea 28 ea likokoana-hloko tsa phefumoloho, le mefuta ea 29 ea mycobacteria e se nang tuberculous (joalokaha ho bontšitsoe Lethathamong la 3).2) Ha ho na karabelo ha u sebelisa khiti ho bona libaka tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le meriana tsa rifampicin le isoniazid e hlokolosi ea Mycobacterium tuberculosis (joalokaha ho bontšitsoe ho Lethathamo la 4).3) Lintho tse tloaelehileng tse sitisang lisampole tse lokelang ho lekoa, tse kang rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), dem pyrazinamide (45mg/L) . (20mg/L) lithethefatsi, ha li na phello ho sephetho sa tlhahlobo ea kit. |
| Lisebelisoa tse sebetsang | SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.), Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 |
Kakaretso ea Tharollo ea PCR
