Enterovirus Universal
Lebitso la sehlahisoa
HWTS-EV001- Sete ea ho Fumana Sesosa sa Enterovirus Universal Nucleic Acid (Fluorescence PCR)
Thuto ea mafu a seoa
Lefu la molomo oa letsoho ke lefu le tšoaetsanoang le bakoang ke li-enterovirus (EV). Hona joale, ho fumanoe mefuta e 108 ea li-serotype tsa li-enterovirus, tse arotsoeng ka lihlopha tse 'ne: A, B, C le D. Har'a tsona, enterovirus EV71 le CoxA16 ke tsona likokoana-hloko tse ka sehloohong. Lefu lena le hlaha haholo-holo ho bana ba ka tlase ho lilemo tse 5, 'me le ka baka herpes matsohong, maotong, molomong le likarolong tse ling. Palo e nyane ea bana e tla ba le mathata a kang myocarditis, pulmonary edema, le aseptic meningoencephalitis.
Mokero
| FAM | EV RNA |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Swab ea oropharyngeal,Mokelikeli oa Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 500/mL |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500/7500 Mekhoa ea PCR e Potlakileng ea Nako ea 'Nete, QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Sete e Khothaletsang ea ho Ntša: Sete ea DNA/RNA e Akaretsang ea Macro le Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), e lokela ho ntšoa ka tieo ho latela litaelo. Bophahamo ba sampole ke 200 μL, bophahamo bo khothaletsoang ba elution ke 80µL.
Khetho ea 2.
Sete e khothaletsoang ea ho ntša: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), e lokela ho ntšoa ka tieo ho latela litaelo.
Khetho ea 3.
Sete e khothaletsoang ea ho ntša: QIAamp Viral RNA Mini Kit (52904) kapa Nucleic Acid Extraction kapa Purification Kit (YDP315-R), e lokela ho ntšoa ho latela litaelo ka tieo. Bophahamo ba sampole ke 140 μL, bophahamo ba elution bo khothaletsoang ke 60µL.
