Enterovirus Universal
Lebitso la sehlahisoa
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Lefu la leoto-molomo ke lefu le tšoaetsanoang le bakoang ke enteroviruses (EV). Hona joale, ho fumanoe mefuta e 108 ea li-serotypes tsa enteroviruses, tse arotsoeng ka lihlopha tse 'nè: A, B, C le D. Har'a tsona, enterovirus EV71 le CoxA16 ke likokoana-hloko tse ka sehloohong. Lefu lena hangata le hlaha ho bana ba ka tlase ho lilemo tse 5, 'me le ka baka herpes matsohong, maotong, molomong le likarolong tse ling. Palo e nyenyane ea bana e tla ba le mathata a kang myocarditis, pulmonary edema, le aseptic meningoencephalitis.
Channel
| FAM | EV RNA |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Oropharyngeal swab,Mokelikeli oa Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Likopi / mL |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500/7500 Fast Real-Time PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Khothaletsa Kit Extraction: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3017-32), HWTS-300 ka tieo ho latela litaelo. Bolumo ea sampole ke 200 μL, bophahamo ba molumo o khothaletsoang ke 80µL.
Khetho ea 2.
Setsi sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), se lokela ho ntšoa ka thata ho latela litaelo.
Khetho3.
Setsi sa ho ntša se khothalelitsoeng: QIAamp Viral RNA Mini Kit (52904) kapa Nucleic Acid Extraction or Purification Kit (YDP315-R), se lokela ho ntšoa ho latela litaelo. Bolumo ea sampole ke 140 μL, bophahamo ba molumo o khothaletsoang ke 60µL.
