Enterovirus 71 (EV71)
Lebitso la sehlahisoa
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Lefu la leoto-molomo ke lefu le tšoaetsanoang le bakoang ke enteroviruses (EV). Hona joale, ho fumanoe mefuta e 108 ea li-serotypes tsa enteroviruses, tse arotsoeng ka lihlopha tse 'nè: A, B, C le D. Har'a tsona, enterovirus EV71 le CoxA16 ke likokoana-hloko tse ka sehloohong. Lefu lena hangata le hlaha ho bana ba ka tlase ho lilemo tse 5, 'me le ka baka herpes matsohong, maotong, molomong le likarolong tse ling. Palo e nyenyane ea bana e tla ba le mathata a kang myocarditis, pulmonary edema, le aseptic meningoencephalitis.
Channel
| FAM | EV71 |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Oropharyngeal swab,Mokelikeli oa Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Likopi / mL |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Litsamaiso tsa PCR tsa Nako ea 480 LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), 'me ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso. Mehlala e ntšitsoeng ke li-swabs tsa oropharyngeal kapa lisampole tsa mokelikeli oa herpes ho tsoa ho bakuli ba bokelletsoeng setšeng. Kenya li-swabs tse bokelletsoeng ho Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ka kotloloho, vortex 'me u kopanye hantle, beha mocheso oa kamore ka metsotso e 5, u li ntše ebe u li khelosa ho kopanya hantle, ho fumana RNA ea sampole ka' ngoe.
Khetho ea 2.
Sehlahisoa se khothalelitsoeng sa ho ntša: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid (0HCTS-30) HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., mme ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso. Bolumo ea sampole e ntšitsoeng ke 200µL, 'me bophahamo ba modumo e khothaletsoang ke 80µL.
Khetho3.
Sehlahisoa sa ho ntša se khothalelitsoeng: QIAamp Viral RNA Mini Kit (52904) ka QIAGEN kapa TIANamp Virus DNA/RNA Kit (YDP315-R), 'me mokha o lokela ho etsoa ho latela litaelo tse sebelisoang. Bolumo ea sampole e ntšitsoeng ke 140μL, 'me molumo o khothaletsoang oa elution ke 60µL.
