Enterovirus 71 (EV71)
Lebitso la sehlahisoa
HWTS-EV003- Enterovirus 71 (EV71) Kit ea ho Fumana Asiti ea Nyutlelie (Fluorescence PCR)
Thuto ea mafu a seoa
Lefu la molomo oa letsoho ke lefu le tšoaetsanoang le bakoang ke li-enterovirus (EV). Hona joale, ho fumanoe mefuta e 108 ea li-serotype tsa li-enterovirus, tse arotsoeng ka lihlopha tse 'ne: A, B, C le D. Har'a tsona, enterovirus EV71 le CoxA16 ke tsona likokoana-hloko tse ka sehloohong. Lefu lena le hlaha haholo-holo ho bana ba ka tlase ho lilemo tse 5, 'me le ka baka herpes matsohong, maotong, molomong le likarolong tse ling. Palo e nyane ea bana e tla ba le mathata a kang myocarditis, pulmonary edema, le aseptic meningoencephalitis.
Mokero
| FAM | EV71 |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Swab ea oropharyngeal,Mokelikeli oa Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | Likopi tse 500/mL |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Mekhoa ea PCR ea Nako ea 'Nete ea 480 Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Reagent e kgothalletswang ho ntsha: Reagent ya Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), mme ho ntsha ho lokela ho etswa ho ya ka taelo ya tshebediso. Disampole tse ntshitsweng ke diswab tsa oropharyngeal kapa disampole tsa metsi a herpes tse tswang ho bakudi ba bokelletsweng sebakeng seo. Kenya diswab tse bokelletsweng ho Reagent ya Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ka ho toba, o di ntshe ka vortex mme o di kopanye hantle, di behe mochesong wa kamore metsotso e 5, di ntshe ebe o di fetola ho di kopanya hantle, ho fumana RNA ya sampole ka nngwe.
Khetho ea 2.
Reagent e kgothalletswang ya ho ntsha: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., mme ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso. Bophahamo ba sampole e ntshitsweng ke 200µL, mme bophahamo ba elution bo kgothaletswang ke 80µL.
Khetho ea 3.
Reagent e kgothalletswang ho ntsha: QIAamp Viral RNA Mini Kit (52904) ka QIAGEN kapa TIANamp Virus DNA/RNA Kit (YDP315-R), mme ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso. Bophahamo ba sampole e ntshitsweng ke 140μL, mme bophahamo ba elution e kgothalletswang ke 60µL.
