Mefuta e 12 ea Pathogen e Phefumolohang
Lebitso la sehlahisoa
HWTS-RT071A Mefuta e 12 ea Phefumoloho ea Nucleic Acid Detection Kit (Fluorescence PCR)
Channel
| Channel | hu12Reaction Buffer A | hu12Reaction Buffer B | hu12Reaction Buffer C | hu12Rection Buffer D |
| FAM | SARS-CoV-2 | KHABANE | HPIV Ⅰ | HRV |
| VIC/HEX | Taolo ea ka hare | Taolo ea ka hare | HPIV Ⅱ | Taolo ea ka hare |
| CY5 | IFV A | MP | HPIV Ⅲ | / |
| ROX | IFV B | RSV | HPIV Ⅳ | HMPV |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2:300 Likopi/mLkokoana-hloko ea ntaramane ea B: 500 Likopi / mLkokoana-hloko ea ntaramane A: 500 Copies/mL Adenovirus: 500 Likopi / mL mycoplasma pneumoniae: 500 Likopi / mL ho hema syncytial kokoana-hloko: 500 Likopi / mL, parainfluenza virus (Ⅰ, Ⅱ, Ⅲ, Ⅳ):500 Likopi/mL Rhinovirus: 500 Likopi / mL metapneumovirus ea motho: 500 Copies/mL |
| Tse khethehileng | Boithuto ba cross-reactivity bo bonts'a hore ha ho na cross-reactivity lipakeng tsa kit ena le enterovirus A, B, C, D, kokoana ea epstein-barr, kokoana-hloko ea maselese, cytomegalovirus ea batho, rotavirus, norovirus, kokoana-hloko ea mumps, varicella-herpes zoster virus, bordetella pertussis, streptococcus pyogenesis, candida fucogenesis, mycogenesis albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans le human genomic nucleic acid. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Fast Real-Time PCR SystemsQuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd. ) LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR, LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo.
Khetho ea 2.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-0060) Macro & Micro-Test Med-Tech Co., Ltd., ho ntšoa ho lokela ho etsoa ho latela litaelo tse tiileng. Molumo o khothaletsoang oa elution ke 80μL.
Khetho ea 3.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction or Purification Kit (YDP315) ke Tiangen Biotech(Beijing) Co.,Ltd., ho ntšoa ho lokela ho etsoa ho latela litaelo tse tiileng. Molumo o khothaletsoang oa elution ke 100μL.
