Mefuta e 11 ea Pathogen ea ho Phefumoloha
Lebitso la sehlahisoa
HWTS-RT162A Mefuta e 11 ea Sete ea ho Fumana Pathogen Nucleic Acid ea Pathogen ea ho Phefumoloha(Phatsimo ea PCR ea Fluorescence)
Mokero
| 靶标 | Mokero | |||
| 核酸反应液A | 核酸反应液B | 核酸反应液C | 核酸反应液D | |
| HI | Smet | MP | Leoto | FAM |
| SP | PA | Bp | / | CY5 |
| KPN | ABA | Bpp | Cpn | ROX |
| 内参 | 内参 | 内参 | 内参 | VIC/HEX |
Thuto ea mafu a seoa
Tšoaetso ea phefumoloho ke sehlopha sa bohlokoa sa mafu a behang bophelo bo botle ba batho kotsing e kholo, 'me lithuto li bontšitse hore boholo ba tšoaetso ea phefumoloho e bakoa ke likokoana-hloko tsa baktheria kapa tsa vaerase tse tšoaetsang motho ea li amohelang hammoho, e leng se lebisang ho matla a lefu lena esita le lefu, kahoo ho hlakisa tšoaetso ho lumella kalafo e reretsoeng 'me ho ntlafatsa sekhahla sa ho phela ha mokuli.[1,2].
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sekhohlela |
| Ct | HI, SP, KPN, PA, ABA, Smet: Ct≤33BP, Bpp, MP, Cpn, Leoto: Ct≤38 |
| CV | <5.0% |
| LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasaeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia le Legionellapneumophila: 1000 CFU/mL； Bordetella pertussis le Bacillus parapertussis: 500 CFU/mL; Mycoplasma pneumoniae le Chlamydia pneumoniae: likopi tse 200/mL. |
| Ho hlaka | Liphetho tsa liteko tsa ho kopanya lisele li bontšitse hore ho ne ho se na karabelo e kopaneng pakeng tsa kit ena le Cytomegalovirus, mofuta oa 1 oa vaerase ea Herpes simplex, vaerase ea Varicella-zoster, vaerase ea Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, mefuta e fokolitsoeng ea Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii le di-nucleic acid tsa genomic tsa batho. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Kit ea Macro & Micro-Test General DNA/RNA (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 200µL eafisiolojie tšele letsoai ho ea ho precipitate e sebetsitsoeng, 'me mehato e latelang e lokela ho etsoa ho latela litaelo. Bophahamo bo khothaletsoang ba elution ke 100 µL.
