Mefuta e 11 ea Pathogen e Phefumolohang
Lebitso la sehlahisoa
HWTS-RT162A 11 Mefuta ea Phefumoloho ea Pathogen Nucleic Acid Detection Kit(Fluorescence PCR)
Channel
| 靶标 | Channel | |||
| 核酸反应液A | 核酸反应液B | 核酸反应液C | 核酸反应液D | |
| HI | Smet | MP | Leoto | FAM |
| SP | PA | Bp | / | CY5 |
| KPN | ABA | Bpp | Cpn | ROX |
| 内参 | 内参 | 内参 | 内参 | VIC/HEX |
Epidemiology
Ts'oaetso ea phefumoloho ke sehlopha sa bohlokoa sa mafu a bakang tšokelo e kholo ho bophelo ba batho, 'me liphuputso li bontšitse hore mafu a mangata a phefumoloho a bakoa ke likokoana-hloko tsa baktheria kapa tsa kokoana-hloko tse tšoaetsang moeti, e leng se lebisang ho matla a eketsehileng a lefu lena esita le lefu, kahoo ho hlakisa pathogen ho lumella phekolo e lebisitsoeng le ho ntlafatsa lebelo la ho pholoha ha mokuli.[1,2].
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sekhohlela |
| Ct | HI,SP,KPN,PA,ABA,Smet:Ct≤33BP, Bpp,MP,Cpn,Leg:Ct≤38 |
| CV | <5.0% |
| LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasaeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia le Legionellapneumophila: 1000 CFU/mL; Bordetella pertussis le Bacillus parapertussis: 500 CFU / mL; Mycoplasma pneumoniae le Chlamydia pneumoniae: likopi tse 200/mL. |
| Tse khethehileng | Liphetho tsa tlhahlobo ea cross-reactivity li bonts'itse hore ho ne ho se na karabelo lipakeng tsa kit ena le Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella-zoster virus, Camophilus influenzae Lefuba la Mycobacterium, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stekhomalcoccus epidermidis, Coccus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Cocomal bacterium Bursemonace. striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlaciimic nucleic acid e chesa batho. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co.,µ0 Eketsa L20fisiolojisaline ho precipitate e entsoeng, 'me mehato e latelang e lokela ho etsoa ho latela litaelo. Bophahamo bo khothaletsoang ba tlhahiso ke 100 µL.
