Kokoana ea Zika
Lebitso la sehlahisoa
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Kokoana-hloko ea Zika ke ea mofuta oa Flaviviridae, ke kokoana-hloko ea RNA e thipa e le 'ngoe e bophara ba 40-70nm. E na le enfelopo, e na le li-nucleotide tse 10794, 'me e kenyelletsa 3419 amino acid. Ho ea ka genotype, e arotsoe ka mofuta oa Afrika le mofuta oa Asia. Lefu la kokoana-hloko ea Zika ke lefu le tšoaetsanoang le itšebetsang le bakoang ke kokoana-hloko ea Zika, e fetisoang haholo ka ho longoa ke menoang ea Aedes aegypti. Likarolo tsa kliniki haholo-holo ke feberu, lekhopho, arthralgia kapa conjunctivitis, 'me ha se hangata e bolaeang. Ho ea ka Mokhatlo oa Lefatše oa Bophelo, lesea la lesea le bitsoang microcephaly le Guillain-Barre syndrome (Guillain-Barré syndrome) li ka amahanngoa le tšoaetso ea kokoana-hloko ea Zika.
Channel
| FAM | Zika kokoana-hloko ea nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤30 ℃ & e sirelelitsoe khanyeng |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | serum e ncha |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Likopi / mL |
| Tse khethehileng | Sebelisa kit ho bona lisampole tsa serum tse nang le kokoana-hloko ea Zika, 'me liphello li mpe. Liphetho tsa liteko tsa tšitiso li bonts'a hore ha khatello ea bilirubin ka serum e sa fete 168.2μmol/ml, khatello ea hemoglobin e hlahisoang ke hemolysis ha e fete 130g/L, khatello ea lipid maling ha e fete 65mmol/ml, kakaretso ea IgG ka serum ha e fete 5mg/mL, ha ho na kokoana-hloko ea dengue kapa kokoana-hloko ea Zika. Kokoana ea Hepatitis A, kokoana-hloko ea Hepatitis B, kokoana-hloko ea Hepatitis C, kokoana-hloko ea Herpes, kokoana-hloko ea Eastern equine encephalitis, Hantavirus, kokoana-hloko ea Bunya, kokoana-hloko ea West Nile le lisampole tsa batho ba genomic serum li khethoa bakeng sa tlhahlobo ea cross-reactivity, 'me liphetho li bontša hore ha ho na karabelo ea sefapano pakeng tsa kit ena le likokoana-hloko tse boletsoeng ka holimo. |
| Lisebelisoa tse sebetsang | ABI 7500 Real-Nako PCR SystemsABI 7500 Fast Real-Nako PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
QIAamp Viral RNA Mini Kit(52904), Nucleic Acid Extraction or Purification Reagent(YDP315-R) ke Tiangen Biotech(Beijing) Co.,Ltd.Ho ntšoae lokela ho ntšoa ho ea ka litaelo tsa ho ntšoa, 'me molumo o khothaletsoang oa ho ntšoa ke 140 μL le molumo o khothaletsoang oa elution ke 60 μL.
Khetho ea 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ho ntšoa ho lokela ho ntšoa ho latela litaelo. Bolumo ea sampole ea ho ntšoa ke 200 μL, 'me bophahamo ba molumo o khothaletsoang ke 80μL.
