Kokoana ea Zika
Lebitso la sehlahisoa
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Kokoana-hloko ea Zika ke ea mofuta oa Flaviviridae, ke kokoana-hloko ea RNA e thipa e le 'ngoe e bophara ba 40-70nm.E na le enfelopo, e na le li-nucleotide tse 10794, 'me e kenyelletsa 3419 amino acid.Ho ea ka genotype, e arotsoe ka mofuta oa Afrika le mofuta oa Asia.Lefu la kokoana-hloko ea Zika ke lefu le tšoaetsanoang le itšebetsang le bakoang ke kokoana-hloko ea Zika, e fetisoang haholo ka ho longoa ke menoang ea Aedes aegypti.Likarolo tsa kliniki haholo-holo ke feberu, lekhopho, arthralgia kapa conjunctivitis, 'me ha se hangata e bolaeang.Ho ea ka Mokhatlo oa Lefatše oa Bophelo, lesea la lesea le bitsoang microcephaly le lefu la Guillain-Barre (Guillain-Barré syndrome) le ka amahanngoa le tšoaetso ea kokoana-hloko ea Zika.
Channel
| FAM | Zika kokoana-hloko ea nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤30 ℃ & e sirelelitsoe khanyeng |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | serum e ncha |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 ng/μL |
| Tse khethehileng | Liphetho tsa liteko tse fumanoeng ke kit ena li ke ke tsa angoa ke hemoglobin (<800g/L), bilirubin (<700μmol/L), le lipids/triglycerides ea mali (<7mmol/L) maling. |
| Lisebelisoa tse sebetsang | ABI 7500 Real-Nako PCR Systems ABI 7500 Fast Real-Nako PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler BioRad CFX96 Real-Nako PCR System, BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
QIAamp Viral RNA Mini Kit(52904), Nucleic Acid Extraction or Purification Reagent(YDP315-R) ke Tiangen Biotech(Beijing) Co.,Ltd.Ho ntšoae lokela ho ntšoa ho ea ka litaelo tsa ho ntšoa, 'me molumo o khothaletsoang oa ho ntša ke 140 μL le molumo o khothaletsoang oa elution ke 60 μL.
Khetho ea 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ho ntšoa ho lokela ho ntšoa ho latela litaelo.Bolumo ea sampole ea ho ntšoa ke 200 μL, 'me bophahamo ba molumo o khothaletsoang ke 80μL.
