Vaerase ea Zika
Lebitso la sehlahisoa
Kiti ea ho Fumana Asiti ea Nyutlelie ea HWTS-FE002 ea Virus ea Zika (Fluorescence PCR)
Setifikeiti
CE
Thuto ea mafu a seoa
Vaerase ea Zika ke ea mofuta oa Flaviviridae, ke vaerase ea RNA e nang le khoele e le 'ngoe e nang le bophara ba 40-70nm. E na le enfelopo, e na le li-nucleotide tse 10794, 'me e na le li-amino acid tse 3419. Ho ea ka mofuta oa genotype, e arotsoe ka mofuta oa Afrika le mofuta oa Asia. Lefu la vaerase ea Zika ke lefu le tšoaetsanoang le iketsang le bakoang ke vaerase ea Zika, e fetisoang haholo-holo ka ho longoa ke menoang ea Aedes aegypti. Litšobotsi tsa kliniki ke haholo-holo feberu, lekhopho, arthralgia kapa conjunctivitis, 'me hangata ha e bolaee. Ho ea ka Mokhatlo oa Lefatše oa Bophelo, microcephaly ea masea le Guillain-Barre syndrome (Guillain-Barré syndrome) li ka amahanngoa le tšoaetso ea vaerase ea Zika.
Mokero
| FAM | Asiti ea nucleic ea kokoana-hloko ea Zika |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤30℃ & e sirelelitsoe khanyeng |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | serum e foreshe |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Likopi tse 500/mL |
| Ho hlaka | Sebelisa kit ho fumana disampole tsa serum tse nang le kokoana-hloko ea Zika e seng ntle, 'me liphetho li mpe. Liphetho tsa liteko tsa tšitiso li bontša hore ha mahloriso a bilirubin serum a sa fete 168.2μmol/ml, mahloriso a hemoglobin a hlahisoang ke hemolysis ha a fete 130g/L, mahloriso a lipid maling a sa fete 65mmol/ml, mahloriso a IgG ka kakaretso serumng ha a fete 5mg/mL, ha ho na phello kokoana-hlokong ea dengue, kokoana-hloko ea Zika kapa chikungunya. Kokoana-hloko ea Hepatitis A, kokoana-hloko ea Hepatitis B, kokoana-hloko ea Hepatitis C, kokoana-hloko ea Herpes, kokoana-hloko ea Eastern equine encephalitis, Hantavirus, kokoana-hloko ea Bunya, kokoana-hloko ea West Nile le lisampole tsa serum ea genomic ea batho li khethiloe bakeng sa teko ea ho kopanya lisele, 'me liphetho li bontša hore ha ho na karabelo e kopaneng pakeng tsa kit ena le likokoana-hloko tse boletsoeng ka holimo. |
| Lisebelisoa tse Sebetsang | Mekhoa ea PCR ea ABI 7500 ea Nako ea SebeleMekhoa ea PCR e Potlakileng ea ABI 7500 ea Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Kit e nyane ea RNA ea Viral ea QIAamp (52904), Nucleic Acid Extract kapa Reagent ea ho Hloekisa (YDP315-R) ke Tiangen Biotech(Beijing) Co.,Ltd.Ho ntšoae lokela ho ntšoa ho latela litaelo tsa ho ntša, 'me bophahamo bo khothaletsoang ba ho ntša ke 140 μL 'me bophahamo bo khothaletsoang ba ho tlosa ke 60 μL.
Khetho ea 2.
Sete ea Macro & Micro-Test Viral DNA/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ho ntšoa ho lokela ho ntšoa ho latela litaelo. Bophahamo ba sampole ea ho ntšoa ke 200 μL, 'me bophahamo bo khothaletsoang ba ho hlakisa ke 80μL.
