SARS-CoV-2/ntara ea ntaramane A /ntara ea ntaramane B

SARS-CoV-2/ntara ya sefuba A /ntara ya sefuba B Setšoantšo se Hlahisitsoeng

Tlhaloso e Khutšoanyane:

Sete ena e loketse ho lemoha SARS-CoV-2, ntaramane A le ntaramane B nucleic acid ka hare ho in vitro ea swab ea nasopharyngeal le disampole tsa swab ea oropharyngeal hore na ke batho bafe ba neng ba belaelloa hore ba na le tšoaetso ea SARS-CoV-2, ntaramane A le ntaramane B. E ka boela ea sebelisoa maemong a belaelloang a pneumonia le linyeoe tsa sehlopha tse belaelloang le bakeng sa ho lemoha le ho khetholla SARS-CoV-2, ntaramane A le ntaramane B nucleic acid ka har'a swab ea nasopharyngeal le disampole tsa swab ea oropharyngeal ea tšoaetso e ncha ea Coronavirus maemong a mang.

Romella imeile ho rona

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

Kiti e Kopaneng ea ho Lemoha ea HWTS-RT148-SARS-CoV-2/Influenza A /Influenza B Nucleic Acid (Fluorescence PCR)

Mokero

Lebitso la Mokero	Motsoako oa PCR 1	Motsoako oa PCR 2
Mokero oa FAM	Jini ea ORF1ab	IVA
Mokero oa VIC/HEX	Taolo ea ka hare	Taolo ea ka hare
Mokero oa CY5	Jini ea N	/
Mokero oa ROX	Jini ea E	IVB

Li-parameter tsa Tekheniki

Polokelo	-18℃
Nako ea ho phela	Likhoeli tse 12
Mofuta oa Sampole	li-swab tsa nasopharyngeal le li-swab tsa oropharyngeal
Sepheo	Lipheo tse tharo tsa SARS-CoV-2 (lijini tsa Orf1ab, N le E)/feberu A /feberu B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2: Likopi tse 300/mL Vaerase ea ntaramane A: Likopi tse 500/mL Vaerase ea ntaramane ea B: Likopi tse 500/mL
Ho hlaka	a) Liphetho tsa liteko tse fapaneng li bontšitse hore kit e ne e tsamaisana le kokoana-hloko ea batho SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, kokoana-hloko ea phefumoloho ea syncytial A le B, kokoana-hloko ea parainfluenza 1, 2 le 3, rhinovirusA, B le C, adenovirus 1, 2, 3, 4, 5, 7 le 55, human metapneumovirus, enterovirus A, B, C le D, human cytoplasmic pulmonary virus, kokoana-hloko ea EB, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata Ha hoa ka ha e-ba le karabelo e kopaneng pakeng tsa Pneumocystis yersini le Cryptococcus neoformans. b) Bokhoni ba ho thibela tšitiso: khetha mucin (60mg/mL), 10% (V/V) mali a motho, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (e nang le sebolaya-dikokwana-hloko) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) le tobramycin (0.6mg/mL). Liphetho li bontšitse hore lintho tse sitisang tse maemong a boletsoeng ka holimo ha li na karabelo ea tšitiso liphellong tsa ho lemoha likokoana-hloko.
Lisebelisoa tse Sebetsang	Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete SLAN^®-96P Mekhoa ea PCR ea Nako ea 'Nete Mekhoa e 5 ea PCR ea QuantStudio™ ea Nako ea 'Nete Leseli la ho Tsamaisa Li-Cycler^®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete