Mycobacterium Tuberculosis Nucleic Acid le Rifampicin Resistance
Lebitso la sehlahisoa
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid le Rifampicin Resistance Detection Kit (Melting Curve)
Setifikeiti
CE
Epidemiology
Mycobacterium tuberculosis , haufinyane joalo ka Tubercle bacillus, TB, ke baktheria ea pathogenic e bakang lefuba. Hona joale, lithethefatsi tse sebelisoang ka mokhoa o tloaelehileng oa ho thibela lefuba li kenyelletsa isoniazid, rifampicin le hexambutol, joalo-joalo Lithethefatsi tse thibelang lefuba tsa moeli oa bobeli li kenyelletsa fluoroquinolones, amikacin le kanamycin, joalo-joalo Lithethefatsi tse ncha tse tsoetseng pele ke linezolid, bedaquiline le delamani, joalo-joalo Leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea li-anti-tuber cell drug le sebopeho sa cellobacterium ea ka. lefuba, lefuba la mycobacterium le hlahisa khanyetso ea lithethefatsi khahlanong le litlhare tse thibelang lefuba, e leng se tlisang mathata a tebileng thibelong le kalafong ea lefuba.
Rifampicin esale e sebelisoa haholo kalafong ea bakuli ba lefuba la matšoafo ho tloha bofelong ba lilemo tsa bo-1970, 'me e na le phello e kholo. E bile khetho ea pele ea ho khutsufatsa chemotherapy ea bakuli ba lefuba la matšoafo. Ho hanyetsa ha Rifampicin ho bakoa haholo-holo ke phetoho ea mofuta oa rpoB. Le hoja litlhare tse ncha tse thibelang lefuba li lula li tsoa, 'me katleho ea bongaka ea bakuli ba lefuba la matšoafo le eona e tsoetse pele ho ntlafala, ho ntse ho e-na le khaello e itseng ea lithethefatsi tse thibelang lefuba,' me ketsahalo ea tšebeliso e sa utloahaleng ea tšebeliso ea lithethefatsi litleleniki e batla e phahame. Ho hlakile hore lefuba la Mycobacterium ho bakuli ba nang le lefuba la matšoafo le ke ke la bolaoa ka ho feletseng ka nako e nepahetseng, e leng se qetellang se lebisa likarolong tse fapaneng tsa ho hanyetsa lithethefatsi 'meleng oa mokuli, ho lelefatsa nako ea lefu lena, le ho eketsa kotsi ea lefu la mokuli.
Channel
| Channel | Likanale le Fluorophores | Reaction Buffer A | Reaction Buffer B | Reaction Buffer C |
| Mokhatlo oa FAM | Motlalehi: FAM, Quencher: Ha ho letho | rpoB 507-514 | rpoB 513-520 | 38KD le IS6110 |
| CY5 Channel | Motlalehi: CY5, Quencher: Ha ho letho | rpoB 520-527 | rpoB 527-533 | / |
| HEX (VIC) Channel | Motlalehi: HEX (VIC), Quencher: Ha ho letho | Taolo ya ka hare | Taolo ya ka hare | Taolo ya ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ Lefifing |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sekhohlela |
| CV | ≤5.0% |
| LoD | lefuba la mycobacterium 50 baktheria/mL mofuta oa hlaha o hananang le rifampicin: 2x103libaktheria/mL phetoho ea homozygous: 2x103libaktheria/mL |
| Tse khethehileng | E lemoha mofuta oa hlaha oa lefuba la mycobacterium le libaka tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le lithethefatsi tse kang katG 315G>C\A, InhA-15C> T, liphetho tsa liteko li bontša hore ha ho na khanyetso ho rifampicin, ho bolelang hore ha ho na cross-reactivity. |
| Lisebelisoa tse Sebetsang: | Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 LightCycler480® Real-Time PCR System |
Phallo ea Mosebetsi
Haeba u sebelisa Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor0 (HTSW30) HTSW30 (HTSW6) & Micro-Test Viral DNA/RNA Column(HWTS-3022-50) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. bakeng sa ho ntšoa, eketsa 200μL ea Taolo e Ntle, taolo e mpe le sampole ea sekhohlela e sebetsitsoeng ho lekoa ka tatellano, 'me u kenye 10μL ho taolo e mpe ea taolo ea ka hare ho taolo e mpe le taolo e ka hare. lekoa, 'me mehato e latelang e lokela ho etsoa ka thata ho latela litaelo tsa ho ntša. Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 100μL.
