Khanyetso ea Mycobacterium Tuberculosis Nucleic Acid le Rifampicin
Lebitso la sehlahisoa
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid le Rifampicin Resistance Detection Kit (Melting Curve)
Setifikeiti
CE
Thuto ea mafu a seoa
Mycobacterium tuberculosis, ka bokhutšoanyane e bitsoang Tubercle bacillus, TB, ke baktheria e bakang lefuba. Hona joale, meriana ea pele e sebelisoang haholo ea ho loantša lefuba e kenyelletsa isoniazid, rifampicin le hexambutol, jj. Meriana ea bobeli ea ho loantša lefuba e kenyelletsa fluoroquinolones, amikacin le kanamycin, jj. Meriana e mecha e ntlafalitsoeng ke linezolid, bedaquiline le delamani, jj. Leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea meriana ea ho loantša lefuba le litšobotsi tsa sebopeho sa lebota la sele la mycobacterium tuberculosis, mycobacterium tuberculosis e hlahisa ho hanyetsa meriana ea ho loantša lefuba, e leng se tlisang liphephetso tse kholo thibelong le kalafong ea lefuba.
Rifampicin e sebelisitsoe haholo kalafong ea bakuli ba lefuba la matšoafo ho tloha bofelong ba lilemo tsa bo-1970, 'me e na le phello e kholo. E bile khetho ea pele ea ho khutsufatsa kalafo ea chemotherapy ea bakuli ba lefuba la matšoafo. Ho hanyetsa Rifampicin ho bakoa haholo ke phetoho ea liphatsa tsa lefutso tsa rpoB. Le hoja meriana e mecha ea ho loantša lefuba e ntse e tsoa khafetsa, 'me katleho ea kliniki ea bakuli ba lefuba la matšoafo le eona e ntse e tsoela pele ho ntlafala, ho ntse ho e-na le khaello e itseng ea meriana ea ho loantša lefuba, 'me ketsahalo ea tšebeliso e sa utloahaleng ea meriana kliniking e phahame haholo. Ho hlakile hore Mycobacterium tuberculosis ho bakuli ba nang le lefuba la matšoafo e ke ke ea bolaoa ka botlalo ka nako e loketseng, e leng se qetellang se lebisa maemong a fapaneng a ho hanyetsa meriana 'meleng oa mokuli, se lelefatsa nako ea lefu lena, 'me se eketsa kotsi ea lefu la mokuli.
Mokero
| Mokero | Likanale le Li-fluorophore | Sesebediswa sa ho Buffer sa Karabelo A | Sesebediswa sa ho Buffer sa Karabelo B | Sesebediswa sa ho Buffer sa Karabelo C |
| Mokero oa FAM | Moqolotsi oa litaba: FAM, Moqhomi: Ha ho letho | rpoB 507-514 | rpoB 513-520 | 38KD le IS6110 |
| Mokero oa CY5 | Moqolotsi oa litaba: CY5, Moqhomi: Ha ho letho | rpoB 520-527 | rpoB 527-533 | / |
| Mokero oa HEX (VIC) | Moqolotsi oa litaba: HEX (VIC), Quencher: Ha ho letho | Taolo ea ka hare | Taolo ea ka hare | Taolo ea ka hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ Lefifing |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sekhohlela |
| CV | ≤5.0% |
| LoD | mycobacterium lefuba baktheria tse 50/mL mofuta o hlaha o hanelang rifampicin: 2x103baktheria/mL phetoho ea homozygous: 2x103baktheria/mL |
| Ho hlaka | E lemoha lefuba la mycobacterium la mofuta o hlaha le libaka tsa phetoho ea liphatsa tse ling tsa lefutso tse hanyetsanang le meriana tse kang katG 315G>C\A, InhA-15C> T, liphetho tsa liteko ha li bontše ho hanyetsa rifampicin, ho bolelang hore ha ho na phetoho e ts'oanang. |
| Lisebelisoa tse Sebetsang: | Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea LightCycler480® ea Nako ea 'Nete |
Phallo ea Mosebetsi
Haeba o sebedisa Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022-50) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. bakeng sa ho ntsha, eketsa 200μL ya Positive Control, negative control le compound sputum sampole e sebeditsweng e tla lekwa ka tatellano, mme o kenye 10μL ya taolo ya kahare ka thoko ho Positive Control, negative control le compound sputum sampole e sebeditsweng e tla lekwa, mme mehato e latelang e lokela ho etswa ka thata ho ya ka ditaelo tsa ho ntsha. Bophahamo ba sampole e ntshitsweng ke 200μL, mme bophahamo bo kgothaletswang ba elution ke 100μL.
