Influenza A Virus Universal/H1/H3
Lebitso la sehlahisoa
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
Kokoana ea ntaramane ke mofuta o emelang Orthomyxoviridae. Ke kokoana-hloko e sokelang bophelo ba batho ka ho teba. E ka tšoaetsa moamoheli haholo. Seoa sa sehla se ama batho ba ka bang limilione tse 600 lefatšeng ka bophara 'me se baka mafu a 250,000 ~ 500,000, moo kokoana-hloko ea ntaramane A e leng sesosa se seholo sa tšoaetso le lefu. Kokoana-hloko ea Influenza A ke RNA e nang le khoele e le 'ngoe e mpe. Ho ea ka holim'a eona hemagglutinin (HA) le neuraminidase (NA), HA e ka aroloa ka lihlopha tse 16, NA E arotsoe ka lihlopha tse 9. Har'a likokoana-hloko tsa ntaramane A, mefuta e nyenyane ea likokoana-hloko tsa ntaramane e ka tšoaetsang batho ka ho toba ke: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 le H10N8. Har'a tsona, li-subtypes tsa H1 le H3 li na le likokoana-hloko tse ngata, 'me li tšoaneloa ke tlhokomelo ka ho khetheha.
Channel
| FAM | influenza Mofuta wa universal virus nucleic acid |
| VIC/HEX | ntaramane A H1 mofuta kokoana ea nucleic acid |
| ROX | ntaramane A H3 kokoana-hloko ea nucleic acid |
| CY5 | taolo ya ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | lesela la nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Tse khethehileng | Ha ho cross-reactivity le lisampole tse ling tsa phefumoloho tse kang Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, kokoana-hloko ea Coxsackie, kokoana-hloko ea Echo, Metapnevirus A1/B2 syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, joalo-joalo le DNA ea genomic ea motho. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Nucleic Acid Extraction or Purification Reagent (YDP315-R) ka Tiangen Biotech (Beijing) Co.,Ltd. Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo tsa tšebeliso. Bolumo ea sampole e ntšitsoeng ke 140μL, 'me molumo o khothaletsoang oa elution ke 60μL.
Khetho ea 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo tsa tšebeliso. Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 80μL.
