Kokoana-hloko ea Influenza A Universal/H1/H3
Lebitso la sehlahisoa
Kit ea ho lemoha bongata ba kokoana-hloko ea HWTS-RT012 Influenza A ea Universal/H1/H3 Nucleic Acid Multiplex (Fluorescence PCR)
Thuto ea mafu a seoa
Vaerase ea ntaramane ke mofuta o emelang Orthomyxoviridae. Ke kokoana-hloko e behang bophelo ba batho kotsing e kholo. E ka tšoaetsa moamoheli haholo. Seoa sena sa sehla se ama batho ba ka bang limilione tse 600 lefatšeng ka bophara 'me se baka mafu a 250,000 ~ 500,000, ao ho 'ona kokoana-hloko ea ntaramane A e leng sesosa se seholo sa tšoaetso le lefu. Vaerase ea ntaramane A ke RNA e nang le marapo a le mong a sa pheleng hantle. Ho ea ka bokaholimo ba eona ba hemagglutinin (HA) le neuraminidase (NA), HA e ka aroloa ka mefuta e 16, NA E arotsoe ka mefuta e 9. Har'a livaerase tsa ntaramane A, mefuta e fapaneng ea livaerase tsa ntaramane tse ka tšoaetsang batho ka kotloloho ke: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 le H10N8. Har'a tsona, mefuta e fapaneng ea H1 le H3 e baka mafu haholo, 'me e tšoaneloa ke tlhokomelo e khethehileng.
Mokero
| FAM | Asiti ea nucleic ea kokoana-hloko ea mofuta oa bokahohle |
| VIC/HEX | Asiti ea nucleic ea kokoana-hloko ea mofuta oa A H1 |
| ROX | Asiti ea nucleic ea kokoana-hloko ea mofuta oa A oa H3 |
| CY5 | taolo ea ka hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | swab ea nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 500/μL |
| Ho hlaka | Ha ho na ho kopana ha di-reactivity le disampole tse ding tsa ho hema tse kang Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, jj. le human genomic DNA. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Setlolo sa Nucleic Acid kapa Setlolo sa ho Hloekisa (YDP315-R) sa Tiang Biotech (Beijing) Co., Ltd. Ho ntsha ho lokela ho etswa ka thata ho latela ditaelo tsa tshebediso. Bophahamo ba sampole e ntshitsweng ke 140μL, mme bophahamo bo kgothaletswang ba ho hlatswa ke 60μL.
Khetho ea 2.
Sete ea Macro & Micro-Test Viral DNA/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ho ntšoa ho lokela ho etsoa ka tieo ho latela litaelo tsa tšebeliso. Bophahamo ba sampole e ntšitsoeng ke 200μL, 'me bophahamo bo khothaletsoang ba elution ke 80μL.
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