Phetoho ea liphatsa tsa lefutso tsa TEL-AML1 ea Motho
Lebitso la sehlahisoa
HWTS-TM016 Human TEL-AML1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Thuto ea mafu a seoa
Leukemia e matla ea lymphoblastic (ALL) ke lefu le atileng haholo bongoaneng. Lilemong tsa morao tjena, leukemia e matla (AL) e fetohile ho tloha mofuteng oa MIC (morphology, immunology, cytogenetics) ho ea mofuteng oa MICM (tlatsetso ea liteko tsa baeloji ea limolek'hule). Ka 1994, ho ile ha fumanoa hore ho kopana ha TEL bongoaneng ho bakoa ke phetoho e sa reroang ea chromosomal t(12;21)(p13;q22) ho B-lineage acute lymphoblastic leukemia (ALL). Ho tloha ha ho sibolloa gene ea AML1 fusion, gene ea TEL-AML1 fusion ke tsela e molemohali ea ho ahlola tebello ea bana ba nang le leukemia e matla ea lymphoblastic.
Mokero
| FAM | Jini ea motsoako oa TEL-AML1 |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | sampole ea moko oa masapo |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | Likopi tse 1000/mL |
| Ho hlaka | Ha ho na ho kopana ha dijini pakeng tsa dikiti le dijini tse ding tsa ho kopanya tse kang dijini tsa ho kopanya tsa BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, PML-RARa. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Sete ea ho ntša RNA ea mali a hloekileng ea RNAprep (DP433).
