Kit ea ho Fumana Liphetoho tsa Gene 29 ea Motho ea EGFR (Fluorescence PCR)-RUO

Kankere ea matšoafo e fetohile sesosa se seholo sa mafu a kankere lefatšeng ka bophara, e leng se behang bophelo bo botle ba batho kotsing e kholo. Kankere ea matšoafo e seng ea lisele tse nyane e ikarabella bakeng sa hoo e ka bang 80%-85% ea bakuli ba kankere ea matšoafo. EGFR hajoale ke sepheo sa bohlokoa ka ho fetisisa sa limolek'hule bakeng sa kalafo ea kankere ea matšoafo e seng ea lisele tse nyane. Phosphorylation ea EGFR e ka khothaletsa kholo ea lisele tsa hlahala, phapang, tlhaselo, metastasis, anti-apoptosis, le ho khothaletsa angiogenesis ea hlahala. EGFR tyrosine kinase inhibitors (TKI) e ka thibela tsela ea lipontšo tsa EGFR ka ho thibela autophosphorylation ea EGFR, ka hona e thibela ho ata le ho fapana ha lisele tsa hlahala, e khothalletsa apoptosis ea lisele tsa hlahala, e fokotsa angiogenesis ea hlahala, jj., e le ho fihlela phekolo e shebaneng le hlahala [1]. Palo e kholo ea lithuto e bontšitse hore katleho ea kalafo ea EGFR-TKI e amana haufi-ufi le boemo ba phetoho ea liphatsa tsa lefutso tsa EGFR, 'me e ka thibela ka ho khetheha kholo ea lisele tsa hlahala ka phetoho ea liphatsa tsa lefutso tsa EGFR [2,3]. Jini ea EGFR e fumaneha letsohong le lekhutšoanyane la chromosome 7 (7p12), e bolelele bo felletseng ba 200Kb 'me e na le li-exons tse 28. Sebaka sa phetoho ea liphatsa tsa lefutso se fumaneha haholo-holo ho li-exons tse 18 ho isa ho tse 21, li-codon tse 746 ho isa ho tse 753 tse tlosoang ho exon 19 li etsa hoo e ka bang 45% 'me phetoho ea L858R ho exon 21 e etsa hoo e ka bang 40% ho isa ho 45% [4]. Tataiso ea NCCN bakeng sa Tlhahlobo le Kalafo ea Kankere ea Matšoafo ea Lisele tse Nyenyane e bolela ka ho hlaka hore tlhahlobo ea phetoho ea liphatsa tsa lefutso ea EGFR ea hlokahala pele ho tsamaiso ea EGFR-TKI. Kiti ena ea teko e sebelisoa ho tataisa tsamaiso ea meriana ea epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), 'me e fana ka motheo oa meriana e ikhethileng bakeng sa bakuli ba nang le kankere ea matšoafo ea lisele tse nyenyane. Kiti ena e sebelisoa feela bakeng sa ho lemoha liphetoho tse tloaelehileng ho jini ea EGFR ho bakuli ba nang le kankere ea matšoafo ea lisele tse nyenyane. Liphetho tsa liteko ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le motheo feela oa kalafo e ikhethileng ea bakuli. Lingaka li lokela ho nahana ka boemo ba mokuli, matšoao a meriana le kalafo. Karabelo le matšoao a mang a liteko tsa laboratori le lintlha tse ling li sebelisoa ho ahlola ka botlalo liphetho tsa liteko.