Human Cytomegalovirus (HCMV) Nucleic Acid
Lebitso la sehlahisoa
HWTS-UR008A-Human cytomegalovirus (HCMV) nucleic acid kit (Fluorescence PCR)
Epidemiology
Human cytomegalovirus (HCMV) ke setho se nang le genome e kholo ka ho fetisisa lelapeng la herpes virus mme e ka kenyelletsa liprotheine tse fetang 200.HCMV e thibetsoe ka mokhoa o fokolang sebakeng sa eona se amohelang batho, 'me ho ntse ho se na mohlala oa liphoofolo oa tšoaetso ea eona.HCMV e na le potoloho e liehang le e telele ea ho ikatisa ho theha 'mele oa ho kenyelletsa intranuclear, le ho etsa hore ho be le 'mele oa ho kenyelletsa perinuclear le cytoplasmic le ho ruruha ha sele (lisele tse kholo), ka hona lebitso.Ho ea ka mefuta e fapaneng ea genome le phenotype ea eona, HCMV e ka aroloa ka mefuta e sa tšoaneng, eo har'a eona ho nang le mefuta e itseng ea li-antigenic, tseo, leha ho le joalo, li se nang bohlokoa ba kliniki.
Ts'oaetso ea HCMV ke ts'oaetso ea tsamaiso, eo ka mokhoa oa bongaka e amang litho tse ngata, e na le matšoao a rarahaneng le a sa tšoaneng, hangata e khutsitse, 'me e ka etsa hore bakuli ba' maloa ba be le liso tsa litho tse ngata tse akarelletsang retinitis, hepatitis, pneumonia, encephalitis, colitis, monocytosis le thrombocytopenic. purpura.Tšoaetso ea HCMV e tloaelehile haholo 'me e bonahala e ata lefatšeng ka bophara.E atile haholo ho baahi, ka sekhahla sa ts'oaetso ea 45-50% le ho feta 90% linaheng tse tsoetseng pele le tse tsoelang pele, ka ho latellana.HCMV e ka robala 'meleng nako e telele.Hang ha tšireletso ea 'mele e fokotsehile, kokoana-hloko e tla kenngoa ts'ebetsong ho baka mafu, haholo-holo ho tšoaetsoa hangata ho bakuli ba leukemia le bakuli ba transplant,' me e ka baka necrosis ea litho tsa 'mele le ho beha bophelo ba bakuli kotsing maemong a boima.Ntle le ho tsoala lesea le shoeleng, ho senyeheloa ke mpa le ho beleha pele ho nako ka tšoaetso ea intrauterine, cytomegalovirus e ka boela ea baka mathata a congenital, kahoo tšoaetso ea HCMV e khona ho ama tlhokomelo ea bakhachane le ba pelehi le boleng ba baahi.
Channel
FAM | HCMV DNA |
VIC(HEX) | Taolo ea ka hare |
Tekheniki Parameters
Polokelo | Mokelikeli: ≤-18℃ Lefifing |
Shelf-bophelo | Likhoeli tse 12 |
Mofuta oa Mohlala | Mohlala oa Serum, Mohlala oa Plasma |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Likopi/karabelo |
Tse khethehileng | Ha ho na cross-reactivity le kokoana-hloko ea hepatitis B, kokoana-hloko ea hepatitis C, kokoana-hloko ea papilloma ea motho, kokoana-hloko ea herpes simplex mofuta oa 1, herpes simplex virus mofuta oa 2, lisampole tse tloaelehileng tsa serum ea motho, joalo-joalo. |
Lisebelisoa tse Sebetsang: | E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ho ntšoa ho lokela ho ntšoa ho latela litaelo.Bolumo ea sampole ea ho ntšoa ke 200μL, 'me bophahamo ba molumo o khothaletsoang ke 80μL.
Sehlahisoa se khothalelitsoeng: QIAamp DNA Mini Kit (51304), Nucleic Acid Extraction or Purification Reagent(YDP315) by Tiangen Biotech(Beijing) Co.,Ltd.e lokela ho ntšoa ho ea ka litaelo tsa ho ntšoa, 'me bophahamo ba molumo o khothaletsoang ke 200 μL le bophahamo ba molumo o khothaletsoang ke 100 μL.