Bongata ba Liphatsa tsa Lefutso tsa CYP2C9 tsa Motho le VKORC1
Lebitso la sehlahisoa
HWTS-GE014A-Human CYP2C9 le VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)
Setifikeiti
CE/TFDA
Thuto ea mafu a seoa
Warfarin ke sethibela-mafu se nooang se sebelisoang haholo mesebetsing ea bongaka hajoale, seo haholo-holo se reretsoeng ho thibela le ho phekola mafu a thromboembolic. Leha ho le joalo, warfarin e na le monyetla o fokolang oa kalafo 'me e fapana haholo har'a merabe le batho ka bomong. Lipalo-palo li bontšitse hore phapang ea tekanyo e tsitsitseng ho batho ka bomong e ka ba makhetlo a fetang 20. Ho tsoa mali ho bakoang ke karabelo e mpe ho etsahala ho 15.2% ea bakuli ba nkang warfarin selemo se seng le se seng, bao 3.5% ea bona ba fumanang mali a bolaeang. Lithuto tsa Pharmacogenomic li bontšitse hore polymorphism ea liphatsa tsa lefutso ea enzyme e reriloeng ea VKORC1 le enzyme ea metabolism CYP2C9 ea warfarin ke ntlha ea bohlokoa e amang phapang ea tekanyo ea warfarin. Warfarin ke thibelo e itseng ea vithamine K epoxide reductase (VKORC1), 'me ka hona e thibela tlhahiso ea clotting factor e kenyeletsang vithamine K' me e fana ka anticoagulation. Palo e kholo ea lithuto e bontšitse hore polymorphism ea liphatsa tsa lefutso ea promoter ea VKORC1 ke ntlha ea bohlokoa ka ho fetisisa e amang phapang ea morabe le ea motho ka mong tekanyong e hlokahalang ea warfarin. Warfarin e silwa ke CYP2C9, 'me liphetoho tsa eona li lieha haholo ho fetola mokhoa oa ho sebelisa warfarin. Batho ba sebelisang warfarin ba na le kotsi e kholo (e fetang habeli ho isa ho makhetlo a mararo) ea ho tsoa mali mohatong oa pele oa tšebeliso.
Mokero
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Li-parameter tsa Tekheniki
| Polokelo | Metsi: ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Mali a foreshe a EDTA a sa kang a bokellana maling |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Ho hlaka | Ha ho na karabelo e ts'oanang le tatellano e 'ngoe e tsitsitseng haholo ea genome ea motho (gene ea CYP2C19 ea motho, gene ea RPN2 ea motho); phetoho ea CYP2C9*13 le VKORC1 (3730G>A) ka ntle ho sebaka sa ho lemoha sa kit ena. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e kgothaletswang ya ho ntsha: Kit ya Macro & Micro-Test Viral DNA/RNA (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
