Vaerase ea Hepatitis E
Lebitso la sehlahisoa
Kiti ea ho Fumana Asiti ea Hepatitis E ea HWTS-HP006 (Fluorescence PCR)
Thuto ea mafu a seoa
Vaerase ea Hepatitis E (HEV) ke vaerase ea RNA e bakang mathata a bophelo bo botle lefatšeng ka bophara. E na le mefuta e mengata ea batho ba e amohelang 'me e na le thepa ea ho tšela lithibelo tsa mefuta e fapaneng. Ke e 'ngoe ea likokoana-hloko tsa bohlokoa ka ho fetisisa tsa zoonotic' me e baka kotsi e kholo ho batho le liphoofolo. HEV e fetisoa haholo-holo ka phetiso ea mantle-molomong, 'me e ka boela ea fetisoa ka ho otloloha ka masea kapa mali. Har'a tsona, tseleng ea phetiso ea mantle-molomong, metsi le lijo tse silafalitsoeng ke HEV li ata haholo, 'me kotsi ea tšoaetso ea HEV ho batho le liphoofolo e holimo [1-2].
Mokero
| FAM | Asiti ea nucleic ea HEV |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sesepa sa 'metso |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 500/μL |
| Ho hlaka | Vaerase ea Hepatitis E (HEV) ke vaerase ea RNA e bakang mathata a bophelo bo botle lefatšeng ka bophara. E na le mefuta e mengata ea batho ba e amohelang 'me e na le thepa ea ho tšela lithibelo tsa mefuta e fapaneng. Ke e 'ngoe ea likokoana-hloko tsa bohlokoa ka ho fetisisa tsa zoonotic' me e baka kotsi e kholo ho batho le liphoofolo. HEV e fetisoa haholo-holo ka phetiso ea mantle-molomong, 'me e ka boela ea fetisoa ka ho otloloha ka masea kapa mali. Har'a tsona, tseleng ea phetiso ea mantle-molomong, metsi le lijo tse silafalitsoeng ke HEV li ata haholo, 'me kotsi ea tšoaetso ea HEV ho batho le liphoofolo e holimo [1-2]. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Sistimi ea PCR e Potlakileng ea 7500 ea Biosystems e Potlakileng ea Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1
Seteishene sa DNA/RNA se Akaretsang sa Macro le Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro le Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). E lokela ho ntšoa ho latela litaelo. Bophahamo bo khothaletsoang ba elution ke 80µL.
Khetho ea 2
TIANamp Virus DNA/RNA Kit (YDP315-R) e entsoeng ke Tiangen Biotech (Beijing) Co., Ltd. E lokela ho ntšoa ka tieo ho latela litaelo. Bophahamo ba sampole e ntšitsoeng ke 140μL. Bophahamo bo khothaletsoang ba elution ke 60µL.v
