Vaerase ea Hepatitis A
Lebitso la sehlahisoa
Kiti ea ho Fumana Asiti ea Hepatitis A ea HWTS-HP005 (Fluorescence PCR)
Thuto ea mafu a seoa
Vaerase ea Hepatitis A (HAV) ke sesosa se seholo sa hepatitis e matla ea vaerase. Vaerase ena ke vaerase ea RNA e nang le strand e le 'ngoe e nang le moelelo o motle 'me e tsoa ho mofuta oa Hepadnavirus oa lelapa la Picornaviridae. Vaerase ea Hepatitis A, e fetisetsoang haholo-holo ka tsela ea mantle-molomong, e hanelang mocheso, li-acid le boholo ba metsoako ea tlhaho, e ka phela nako e telele ka har'a shellfish, metsi, mobu kapa mashala a leoatleng [1-3]. E fetisetsoa ka ho ba le lijo kapa metsi a silafetseng, kapa ka ho toba e fetisetsoang ho tloha ho motho ho ea ho motho. Lijo tse amanang le HAV li kenyelletsa li-oyster le li-clam, fragole, raspberries, li-blueberries, li-date, meroho e tala le tamati e omisitsoeng hanyane [4-6].
Mokero
| FAM | Asiti ea nucleic ea HAV |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Metsi: Likhoeli tse 9, Lyophilized: Likhoeli tse 12 |
| Mofuta oa Sampole | Serum/setuloana |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 2/μL |
| Ho hlaka | Sebelisa lisebelisoa tsena ho leka likokoana-hloko tse ling tsa hepatitis tse kang hepatitis B, C, D, E, enterovirus 71, kokoana-hloko ea coxsackie, kokoana-hloko ea Epstein-Barr, norovirus, HIV le genome ea motho. Ha ho na phetoho e 'ngoe. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Quantitative Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Disampole tsa serum
Khetho ea 1.
Seteishene sa DNA/RNA se Akaretsang sa Macro le Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro le Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). E lokela ho ntšoa ho latela litaelo. Bophahamo bo khothaletsoang ba elution ke 80µL.
Khetho ea 2.
TIANamp Virus DNA/RNA Kit (YDP315-R) e hlahisitsoeng ke Tiangen Biotech (Beijing) Co., Ltd. E lokela ho ntšoa ho latela litaelo. Bophahamo ba sampole e ntšitsoeng ke 140μL. Bophahamo bo khothaletsoang ba elution ke 60µL.
2.Mehlala ea Matloana
Seteishene sa DNA/RNA se Akaretsang sa Macro le Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro le Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). E lokela ho ntšoa ho latela litaelo. Bophahamo bo khothaletsoang ba elution ke 80µL.
