Kokoana-hloko ea Influenza/Influenza B Virus Nucleic Acid
Lebitso la sehlahisoa
HWTS-RT193-Fluorescence-Omisitsoeng Virus/Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ho ea ka phapang ea liphatsa tsa lefutso pakeng tsa NP le M, likokoana-hloko tsa ntaramane li ka aroloa ka mefuta e mene: kokoana-hloko ea ntaramane A (IFV A), kokoana-hloko ea B (IFV B), kokoana-hloko ea C (IFV C) le kokoana-hloko ea D (IFV D) . Bakeng sa kokoana-hloko ea ntaramane A, e na le li-host tse ngata le li-serotypes tse rarahaneng, 'me e ka hasana hohle ka ho kopanya liphatsa tsa lefutso le liphetoho tse fetohang. Batho ha ba na tšireletso ea nako e telele ho kokoana-hloko ea ntaramane A, kahoo batho ba lilemo tsohle hangata ba hlaseleha habonolo. Kokoana-hloko ea Influenza A ke likokoana-hloko tse kholo ka ho fetisisa tse bakang mafu a seoa. Bakeng sa kokoana-hloko ea ntaramane ea B, hangata e hasana libakeng tse nyenyane 'me ha joale ha e na mefuta e nyenyane. Tšoaetso ea batho e bakoa haholo-holo ke livaerase tsa ntaramane tsa leloko la B/Yamagata kapa la B/Victoria. Har'a linyeoe tse netefalitsoeng tsa khoeli le khoeli tsa ntaramane linaheng tse 15 tsa sebaka sa Asia-Pacific, tekanyo ea ho fumanoa ha kokoana-hloko ea ntaramane ea B e tloha ho 0 ho ea ho 92%. Ho fapana le kokoana-hloko ea ntaramane A, lihlopha tse itseng tsa batho, tse kang bana le batho ba hōlileng, li kotsing ea ho tšoaroa ke kokoana-hloko ea ntaramane ea B, e ka bakang mathata habonolo, ea baka moroalo o moholo sechabeng ho feta kokoana-hloko ea ntaramane A.
Tekheniki Parameters
| Polokelo | 2-28℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Ho phunya 'metso |
| Ct | IFV A,IFVB Ct≤35 |
| CV | <5.0% |
| LoD | 200 likopi / mL |
| Tse khethehileng | Cross-reactivity: Ha ho na cross-reactivity pakeng tsa kit le Bocavirus, Rhinovirus, Cytomegalovirus, Respiratory syncytial virus, Parainfluenza virus, Epstein-Barr virus, herpes simplex virus, varicella-zoster virus, Mumps virus, Enterovirus, Measles virus, human metapneumovirus, coronavirus ea motho, SARS-virus, SARS, Adenovel, coronavirus MERS-CoV, Rotavirus, Norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis jirovecii, Haemophilus influenzae, Bordetella pertusse, Bordetella pertuscoccus tubercocus gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium le DNA ea motho ea genomic. Teko ea ho kena-kenana: Mucin (60mg/mL), mali a motho (50%), Phenylephrine (2 mg/mL), Oxymetazoline (2mg/mL), sodium chloride (20mg/mL) e nang le 5% preservative, Beclomethasone (20mg/mL), Dexamethasone (20mg/mL) (2mg/mL), Budesonide (1mg/mL), Mometasone (2mg/mL), Fluticasone (2mg/mL), Histamine hydrochloride (5 mg/mL), Benzocaine (10%), Menthol (10%), Zanamivir (20mg/mL), Peramivir (1mg/mL0), Tomycin (1mg/mL) (0.6mg/mL), Oseltamivir (60ng/mL), Ribavirin (10mg/L) e ile ea khethoa bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bonts'a hore lintho tse sitisang likhakanyo tse kaholimo ha li na tšusumetso ea tšitiso ho liphetho tsa liteko tsa kit. |
| Lisebelisoa tse sebetsang | E sebetsa ho mochini oa tlhahlobo oa mofuta oa I: Applied Biosystems 7500 Real-Nako PCR Systems, SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.). E sebetsa ho mochini oa liteko oa Mofuta oa II: EudemonTM AIO800 (HWTS-EQ007) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Phallo ea Mosebetsi
PCR e tloaelehileng
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. IFU ea Kit.
Mochini oa AIO800 kaofela-in-one
