Chlamydia Trachomatis Nucleic Acid
Lebitso la Sehlahiswa
HWTS-UR001A-Chlamydia Trachomatis Nucleic Acid Detection Kit (Fluorescence PCR)
Tšebeliso e reriloeng
Setsi sena se sebelisetsoa ho lemoha Chlamydia trachomatis nucleic acid ka har'a moroto oa banna, lisampole tsa cervical swab ea banna.
Epidemiology
Chlamydia trachomatis (CT) ke mofuta oa likokoana-hloko tsa prokaryotic tse nang le likokoana-hloko ka ho feletseng liseleng tsa eukaryotic.Chlamydia trachomatis e arotsoe ka AK serotypes ho ea ka serotype mokhoa.Urogenital pampitšana mafu a bakoa haholo ke trachoma biological variant DK serotypes, 'me ba batona ba boholo ba bonahatsoa e le urethritis, e ka imoloha ntle le kalafo, empa boholo ba bona e ba a sa foleng, nako le nako e mpefala,' me e ka kopanngoa le epididymitis, proctitis, joalo-joalo. e ka bakoa ke urethritis, cervicitis, joalo-joalo, le mathata a tebileng a salpingitis.
Epidemiology
FAM: Chlamydia trachomatis (CT)·
VIC(HEX): Taolo ea ka hare
PCR Amplification Conditions Setting
Mohato | Lipotoloho | Mocheso | Nako | Bokella Lipontšo tsa Fluorescent kapa Che |
1 | 1 potoloho | 50 ℃ | 5 mets | No |
2 | 1 potoloho | 95℃ | 10metsotso | No |
3 | 40 potoloho | 95℃ | 15 metsotsoana | No |
4 | 58℃ | 31 metsotsoana | Ee |
Tekheniki Parameters
Polokelo | ≤-18℃ Lefifing |
Shelf-bophelo | Likhoeli tse 12 |
Mofuta oa Mohlala | Mahlaseli a banna, Mafu a mosali, Moroto oa banna |
Ct | ≤38 |
CV | 5.0% |
LoD | 400Likopi / mL |
Tse khethehileng | Ha ho na cross-reactivity bakeng sa ho lemoha likokoana-hloko tse ling tse tšoaelitsoeng ke STD ka khithi ena, tse kang Treponema pallidum, Neisseria gonorrhoeae, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, joalo-joalo, tse ka ntle ho sebaka sa ho lemoha. |
Lisebelisoa tse sebetsang | E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |