Chlamydia Trachomatis, Neisseria Gonorrhoeae le Trichomonas vaginalis
Lebitso la sehlahisoa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae le Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chlamydia trachomatis (CT) ke mofuta oa likokoana-hloko tsa prokaryotic tse nang le likokoana-hloko ka ho feletseng liseleng tsa eukaryotic.Chlamydia trachomatis e arotsoe ka AK serotypes ho ea ka serotype mokhoa.Urogenital tšoaetso ea pampitšana hangata e bakoa ke trachoma biological variant DK serotypes, 'me banna ba atisa ho bonahatsoa e le urethritis, e ka imolloang ntle le phekolo, empa boholo ba bona e ba bo sa foleng, nako le nako e mpefala,' me e ka kopanngoa le epididymitis, proctitis, joalo-joalo.
Channel
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Swab ea molomo oa mosali,Swab ea botšehali ba basali,Sekhahla sa botona |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Likopi/mL |
| Tse khethehileng | Ha ho na cross-reactivity le likokoana-hloko tse ling tsa tšoaetso ea STD ka ntle ho sebaka sa ho lemoha ha lisebelisoa tsa tlhahlobo, tse kang Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, joalo-joalo. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa se khothalelitsoeng: Pipette 1mL ea sampole e tla lekoa ho 1.5mL ea DNase/RNase-free centrifuge tube, centrifuge ka 12000rpm ka metsotso e 3, lahla supernatant 'me u boloke pula.Eketsa 200µL ea saline e tloaelehileng ho pula ho emisa hape.Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso.Bolumo ea sampole e ntšitsoeng ke 200µL, 'me bophahamo ba modumo e khothaletsoang ke 80µL.
Khetho ea 2.
Sehlahisoa se khothalelitsoeng sa ho ntša: Nucleic Acid Extraction kapa Purification Kit (YDP302).Ho ntšoa ho lokela ho etsoa ka thata ho latela litaelo tsa tšebeliso.Molumo o khothaletsoang oa elution ke 80µL.
